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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
TLD-3A12, monoclonal
Application:
ELISA, FACS, IHC, WB
Citations:
4
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
TLD-3A12, monoclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
suitability
not suitable for immunohistochemistry (Paraffin)
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Immunogen
Activated, Lewis rat derived microglial cells.
Application
Anti-PECAM-1 Antibody, clone TLD-3A12, azide free detects level of PECAM-1 & has been published & validated for use in ELISA, FC, WB, IH.
Flow Cytometry: 1:50-1:100
Immunohistology: frozen sections (-20°C methanol fixation), not recommended for traditional formalin fixed tissues.
ELISA
Western Blotting: 1:500. MW = 130-140 kDa
Optimal working dilutions must be determined by end user.
Immunohistology: frozen sections (-20°C methanol fixation), not recommended for traditional formalin fixed tissues.
ELISA
Western Blotting: 1:500. MW = 130-140 kDa
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Biochem/physiol Actions
Rat PECAM-1. Antibody gives positive reaction at high dilutions against recombinant rat PECAM-1, confirming specificity.
Physical form
Format: Purified
Purified immunoglobulin by affinity chromatography on Protein G. Liquid in phosphate buffered saline containing no preservatives.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
POSITIVE CONTROL: Activated microglial cells.
POSITIVE CONTROL: Activated microglial cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB1393Z | 04053252505652 |