Key Documents
MAB1613
Anti-Kinesin Antibody, heavy chain, non-neuronal KHC, clone H1
clone H1, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
H1, monoclonal
species reactivity
rat, human, bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KIF5B(3799)
Immunogen
Epitope: heavy chain, non-neuronal KHC
Application
Anti-Kinesin Antibody, heavy chain, non-neuronal KHC, clone H1 detects level of Kinesin & has been published & validated for use in ELISA, IP, RIA, WB, IC, IH.
Immunohistochemistry on paraformaldehyde fixed tissue. Also works for EM.
Immunocytochemistry: paraformaldehyde fixed cells; 5-10′ RT, freshly made 4% PFA; permeabilize with 0.1-.2% triton X-100 in 3% normal serum blocking buffer
Immunoblotting: recognizes 124kDa KHC from non-neuronal samples.
Immunoprecipitation
Inhibition of kinesin mediated transport
EIA/RIA
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: paraformaldehyde fixed cells; 5-10′ RT, freshly made 4% PFA; permeabilize with 0.1-.2% triton X-100 in 3% normal serum blocking buffer
Immunoblotting: recognizes 124kDa KHC from non-neuronal samples.
Immunoprecipitation
Inhibition of kinesin mediated transport
EIA/RIA
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Reacts with the major form of heavy chain of non-neuronal KHC near the N-terminus.
Physical form
Format: Purified
Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl.pH 7.6 with 0.1% sodium azide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sten Strunze et al.
Cell host & microbe, 10(3), 210-223 (2011-09-20)
Many viruses deliver their genomes into the host cell nucleus for replication. However, the size restrictions of the nuclear pore complex (NPC), which regulates the passage of proteins, nucleic acids, and solutes through the nuclear envelope, require virus capsid uncoating
Souvik Sarkar et al.
Traffic (Copenhagen, Denmark), 7(8), 1078-1091 (2006-06-01)
Intracellular trafficking regulates the abundance and therefore activity of transporters present at the plasma membrane. The transporter, Na+-taurocholate co-transporting polypeptide (ntcp), is increased at the plasma membrane upon treatment of cells with cAMP, for which microtubules (MTs) are required and
Wen-Jun Wang et al.
Drug metabolism and disposition: the biological fate of chemicals, 42(1), 62-69 (2013-10-12)
Previous studies identified a family of organic anion transport proteins (OATPs), many of which have C-terminal PDZ binding consensus sequences. In particular, the C-terminal four amino acids of Oatp1a1, a transporter on rat and mouse hepatocytes, comprise a consensus binding
Hongtao Chen et al.
Biophysical journal, 94(4), 1508-1520 (2007-11-06)
The role of cholesterol in the regulation of endosome motility was investigated by monitoring the intracellular trafficking of endocytosed folate receptors (FRs) labeled with fluorescent folate conjugates. Real-time fluorescence imaging of HeLa cells transfected with green fluorescent protein-tubulin revealed that
Jie Zhou et al.
PLoS pathogens, 14(5), e1007055-e1007055 (2018-05-22)
Many viruses, including adenovirus, exhibit bidirectional transport along microtubules following cell entry. Cytoplasmic dynein is responsible for microtubule minus end transport of adenovirus capsids after endosomal escape. However, the identity and roles of the opposing plus end-directed motor(s) remain unknown.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB1613 | 04053252661358 |
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