MAB1910
Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3
ascites fluid, clone 23IIC3, Chemicon®
Synonym(s):
Anti-BSVD2, Anti-ICH, Anti-POREN2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3, ascites fluid, clone 23IIC3, Chemicon®
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
23IIC3, monoclonal
species reactivity
monkey, human, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL4A2(1284)
Analysis Note
Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia
Application
Detect Collagen Type IV α 2 Chain using this Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3 validated for use in ELISA, IH(P) & WB.
Immunohistochemistry: 1:500, acetone fixed, frozen tissue recommended. Weak reactivity on on formalin fixed, paraffin sections (1:100) after treatment with 1% trypsin (20 minutes, room temperature). Or alternatively, 4% PFA fixed, HEIR treated tissue has worked in monkey (Qin, 2003) at 1:200 dilution.
Western blot: 1:500, 170 kDa.
ELISA at 1:200
Optimal working dilutions must be determined by the end user.
Western blot: 1:500, 170 kDa.
ELISA at 1:200
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Biochem/physiol Actions
Recognizes Human type IV collagen, alpha-2 chains.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
170 kDa
Immunogen
Human amniotic type IV collagen
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Liquid.
Unpurified
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Peter Trosan et al.
PloS one, 13(3), e0194820-e0194820 (2018-03-28)
The human amniotic membrane (HAM) is widely used for its wound healing effect in clinical practice, as a feeder for the cell cultivation, or a source of cells to be used in cell therapy. The aim of this study was
Epithelial organization and cyst lumen expansion require efficient Sec13-Sec31-driven secretion.
Townley, AK; Schmidt, K; Hodgson, L; Stephens, DJ
Journal of Cell Science null
Matteo Tagliapietra et al.
European journal of neurology, 30(12), 3834-3841 (2023-08-02)
Biallelic mutation/expansion of the gene RFC1 has been described in association with a spectrum of manifestations ranging from isolated sensory neuro(no)pathy to a complex presentation as cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS). Our aim was to define
Edward R Chu et al.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 30(2-3), 191-201 (2014-02-13)
We have developed a tissue-based model of the human trabecular meshwork (TM) using viable postmortem corneoscleral donor tissue. Two-photon microscopy is used to optically section and image deep in the tissue to analyze cells and extracellular matrix (ECM) within the
D Ohlund et al.
British journal of cancer, 101(1), 91-97 (2009-06-06)
Pancreas cancer is a dreaded disease with high mortality, despite progress in surgical and oncological treatments in recent years. The field is hampered by a lack of good prognostic and predictive tumour biomarkers to be used during follow-up of patients.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB1910 | 04053252617416 |
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