MAB1911
Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8
clone 9F8, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
9F8, monoclonal
Application:
ELISA, ICC, IHC, WB
Citations:
2
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
9F8, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TNC(3371)
Immunogen
Epitope: type III repeats 1-3
Purified human tenascin
Application
Immunohistochemistry at 2 μg/mL on tissues fixed with cold acetone
Immunocytochemistry at 2 μg/mL on U251 cells
Immunoblotting (Western) at 2 μg/mL to detect whole tenascin under reducing conditions
ELISA: 2 x 10-10 M endpoint dilution
Affinity constant Kd= 6.37 x 10-9
Final working dilutions must be determined by end user.
Immunocytochemistry at 2 μg/mL on U251 cells
Immunoblotting (Western) at 2 μg/mL to detect whole tenascin under reducing conditions
ELISA: 2 x 10-10 M endpoint dilution
Affinity constant Kd= 6.37 x 10-9
Final working dilutions must be determined by end user.
This Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8 is validated for use in ELISA, WB, IC, IH for the detection of Tenascin.
Biochem/physiol Actions
Binds to a recombinant fragment of human tenascin containing the fibronectin type III repeats, numbers 1-3.
Physical form
Format: Purified
Liquid in 0.02 M phosphate, 0.25 M Sodium Chloride pH 7.6, with 0.1% Sodium Azide.
Preparation Note
Store at 2 and 8°C for up to 6 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
MOUSE ANTI-HUMAN TENASCIN
James Ozanne et al.
Frontiers in immunology, 14, 1275361-1275361 (2023-12-11)
Serum biomarkers are the gold standard in non-invasive disease diagnosis and have tremendous potential as prognostic and theranostic tools for patient stratification. Circulating levels of extracellular matrix molecules are gaining traction as an easily accessible means to assess tissue pathology.
Investigating Chemokine-Matrix Networks in Breast Cancer: Tenascin-C Sets the Tone for CCL2.
Gschwandtner, et al.
International Journal of Molecular Sciences, 24 (2023)