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MAB1911

Sigma-Aldrich

Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8

clone 9F8, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

9F8, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_000632.3

UniProt accession no.

P11215

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TNC(3371)

Immunogen

Epitope: type III repeats 1-3
Purified human tenascin

Application

Immunohistochemistry at 2 μg/mL on tissues fixed with cold acetone

Immunocytochemistry at 2 μg/mL on U251 cells

Immunoblotting (Western) at 2 μg/mL to detect whole tenascin under reducing conditions

ELISA: 2 x 10-10 M endpoint dilution

Affinity constant Kd= 6.37 x 10-9

Final working dilutions must be determined by end user.
This Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8 is validated for use in ELISA, WB, IC, IH for the detection of Tenascin.

Biochem/physiol Actions

Binds to a recombinant fragment of human tenascin containing the fibronectin type III repeats, numbers 1-3.

Physical form

Format: Purified
Liquid in 0.02 M phosphate, 0.25 M Sodium Chloride pH 7.6, with 0.1% Sodium Azide.

Preparation Note

Store at 2 and 8°C for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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James Ozanne et al.
Frontiers in immunology, 14, 1275361-1275361 (2023-12-11)
Serum biomarkers are the gold standard in non-invasive disease diagnosis and have tremendous potential as prognostic and theranostic tools for patient stratification. Circulating levels of extracellular matrix molecules are gaining traction as an easily accessible means to assess tissue pathology.
Investigating Chemokine-Matrix Networks in Breast Cancer: Tenascin-C Sets the Tone for CCL2.
Gschwandtner, et al.
International Journal of Molecular Sciences, 24 (2023)

Global Trade Item Number

SKUGTIN
MAB191104053252666698

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