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Key Documents

MAB1921

Sigma-Aldrich

Anti-Laminin β1 Antibody, clone 4E10

ascites fluid, clone 4E10, Chemicon®

Synonym(s):

Laminin B1 Chain

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

ascites fluid

clone

4E10, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Immunogen

Purified human laminin

Application

Anti-Laminin β1 Antibody, clone 4E10 detects level of Laminin β1 & has been published & validated for use in ELISA, IH, IP & WB.
ELISA (50% maximal binding to human laminin): >1:1,600,000.

Immunofluorescence

Affinity chromatography

Immunoprecipitation

Immunoblotting

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Biochem/physiol Actions

Reacts with the B1 portion of intact laminin and blocks the neurite stimulating activity of laminin.

Physical form

Liquid.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Abnormal expression of laminin beta 1 chain in skeletal muscle of adult-onset limb-girdle muscular dystrophy.
M Li, D W Dickson, A J Spiro, M Li, D W Dickson, A J Spiro
Archives of Neurology null
Appearance and distribution of laminin A chain isoforms and integrin alpha 2, alpha 3, alpha 6, beta 1, and beta 4 subunits in the developing human small intestinal mucosa.
N Perreault, P H Vachon, J F Beaulieu, N Perreault, P H Vachon, J F Beaulieu
The Anatomical Record null
The osteogenic differentiation of human bone marrow MSCs on HUVEC-derived ECM and ?-TCP scaffold.
Kang, Y; Kim, S; Bishop, J; Khademhosseini, A; Yang, Y
Biomaterials null
Renate Kunert et al.
AIDS research and human retroviruses, 20(7), 755-762 (2004-08-17)
The human monoclonal antibody 4E10 has been generated previously by immortalization of peripheral blood cells from an HIV-1-infected individual. This antibody binds to the linear epitope NWFDIT on gp41 and exhibits exceptional neutralizing activity against a broad spectrum of primary
Human laminin isolated in a nearly intact, biologically active form from placenta by limited proteolysis.
Wewer, U, et al.
The Journal of Biological Chemistry, 258, 12654-12660 (1983)

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