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Merck

MAB1924

Anti-Laminin α5 Antibody, clone 4C7

ascites fluid, clone 4C7, Chemicon®

Synonym(s):

Anti-AA408760, Anti-AA408762, Anti-AI853660, Anti-laminin-511, Anti-mKIAA0533

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4C7, monoclonal
Application:
ELISA, IHC, IP
Citations:
25
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biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

4C7, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... LAMA2(3908)

Application

Anti-Laminin α5 Antibody, clone 4C7 is an antibody against Laminin α5 for use in ELISA, IH & IP.
ELISA (50% maximal binding to human laminin): >1:11,000.

Immunofluorescence - tissue staining pattern is most consistent with that observed for laminin alpha5 in the mouse(Miner et al., 1997)

Affinity chromatography

Immunoprecipitation

Does not work for Western blotting

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with the globular domain of the alpha5 chain of intact laminin and blocks the neurite stimulating activity of laminin. This antibody was originally thought to recognize laminin alpha 1 or A, but further characterization has confirmed its specificity for the laminin alpha 5 chain [see Tiger, C.F. (1997) J. Biol. Chem. 272: 28590].

Physical form

UnPurified mouse ascites containing no preservatives.

Analysis Note

Control
Developing striated muscle tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jamie L Maciaszek et al.
Biophysical journal, 102(5), 1137-1143 (2012-03-13)
Collapse and sudden death in physical training are the most serious complications of sickle cell trait (SCT). There is evidence that erythrocytes in SCT patients aggregate during strenuous exercise, likely because of adhesive interactions with the extracellular matrix (ECM) and
Yuji Sekine et al.
Journal of the American Society of Nephrology : JASN, 20(7), 1586-1596 (2009-05-16)
LAT3 is a Na+-independent neutral l-amino acid transporter recently isolated from a human hepatocellular carcinoma cell line. Although liver, skeletal muscle, and pancreas are known to express LAT3, the tissue distribution and physiologic function of this transporter are not completely
Paolo Giuffrida et al.
Inflammatory bowel diseases, 25(11), 1740-1750 (2019-06-15)
The current methodologies for the identification of therapeutic targets for inflammatory bowel disease (IBD) are limited to conventional 2-dimensional (2D) cell cultures and animal models. The use of 3D decellularized human intestinal scaffolds obtained from surgically resected intestine and engineered