MAB19310
Anti-Aggrecan Antibody
CHEMICON®, mouse monoclonal, AF-28
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Product Name
Anti-Aggrecan Antibody, MMP Cleaved, NT FFGVG neoepitopes, clone AF-28, clone AF-28, Chemicon®, from mouse
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
AF-28, monoclonal
species reactivity
bovine, rat, human, pig, mouse
should not react with
guinea pig, horse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACAN(176)
Analysis Note
Control
Cartilage, neural tube, and brain tissue
Cartilage, neural tube, and brain tissue
Application
Detect Aggrecan using this Anti-Aggrecan Antibody, MMP Cleaved, N-terminus FFGVG neoepitopes, clone AF-28 validated for use in ELISA & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
Inflammation & Autoimmune Mechanisms
ECM Proteins
Inflammation & Autoimmune Mechanisms
Western blot
ELISA
Optimal working dilutions must be determined by the end user.
ELISA
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Mouse anti-aggrecan is a cleavage-site-specific monoclonal antibody for detecting metalloproteinase-derived aggrecan fragments. The antibody recognizes neo-epitopes on polypeptides with N-terminal FFGVG sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with MMPs. By immunoblotting, AF-28 specifically detected G2 fragments derived from an aggrecan G1-G2 substrate digested with stromelysin, collagenase, gelatinase, and matrilysin, but failed to detect G2 fragments obtained from elastase, trypsin, or cathepsin B digests. Undigested G1-G2 was not detected. Competition experiments confirmed that peptides containing internal FFGVG sequences were not detected by the antibody. Clone AF-28 specifically recognizes a neo-epitope on polypeptides with N-terminal FFGVG Sequences. This sequence is found at the N-terminus of aggrecan fragments that have been digested with matrix metalloproteinases.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Aggrecan is also known as aggregating chondroitin sulphate proteoglycan. Aggrecan is the major proteoglycan present in articular cartilage, composing up to 10% of its dry weight. It is responsible for endowing articular with its intrinsic properties of load bearing and compressive forces.
Immunogen
Epitope: N-terminus FFGVG neoepitopes
FFGVGGEEDC-KLH peptide
Physical form
Format: Purified
Liquid in PBS pH 7.4 containing 0.09% sodium azide as a preservative.
Protein A purified
Preparation Note
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Maya Arai et al.
Osteoarthritis and cartilage, 12(8), 599-613 (2004-07-21)
Articular cartilage matrix synthesis and degradation are dynamic processes that must be balanced for proper maintenance of the tissue. In osteoarthritis (OA), this balance is skewed toward degradation and ultimate loss of matrix. The transcriptional and/or activity levels of hundreds
Tohru Takahashi et al.
Stem cells translational medicine, 3(12), 1484-1494 (2014-10-15)
Multipotent mesenchymal stromal cell (MSC) therapy and costimulation blockade are two immunomodulatory strategies being developed concomitantly for the treatment of immunological diseases. Both of these strategies have the capacity to inhibit immune responses and induce regulatory T cells; however, their
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB19310 | 04053252467509 |
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