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Merck

MAB2115

Anti-Cell Surface Antigen of Human Nucleated Erythroid Cells Antibody, clone HAE9

clone HAE9, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Cell Surface Antigen of Human Nucleated Erythroid Cells Antibody, clone HAE9, clone HAE9, Chemicon®, from mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HAE9, monoclonal

species reactivity

human

should not react with

rat, mouse

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunoprecipitation (IP): suitable

isotype

IgMκ

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Related Categories

Application

Immunoprecipitation: 5μg/500μL of cell lysate in 0.5% triton X-100 diluted in PBS with proteinase inhibitors

Flow cytometry: 10μg/mL concentration; (1:100); per 10(e6) cells, 100μL sample volume.

Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-Cell Surface Antigen of Human Nucleated Erythroid Cells Antibody, clone HAE9 is validated for use in FC, IP for the detection of Cell Surface Antigen of Human Nucleated Erythroid Cells.

Biochem/physiol Actions

MAB2115 recognizes a 70 kDa surface protein in about 100% nucleated erythroid cells (erythroblasts, normoblasts, proerythroblasts), 36% of late erythroid committed cells and 70-80% of fetal liver cells. Normal stem cells, early erythroid committed cells and hematopoetic non-erythroid cells do not react with MAB2115. The 70 kDa protein may be associated with an isoform of transferrin specific for red cells. Applicable for (1) immunostaining and enumeration of nucleated erythroid cells in bone marrow, fetal liver or cord blood and (2) immunophenotyping human leukemias. {Mechetner, EB et al (1987) Identification of a human erythroid cell surface antigen by monoclonal antibody HAE9. Exp Hematol. 15(4):355-9}.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human fetal liver cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Purified immunoglobulin via gel filtration from tissue culture supernatants in 20mM PB pH 7.6, 250mM NaCL, with 0.1% sodium azide as a preservative.

Preparation Note

Maintain at 2-8°C for up to 6 months after date of receipt. The product may contain precipitation and microcentrifugation is recommended before use.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MAB211504053252615900

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