MAB2148-C
Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free)
clone P2B1, 1 mg/mL, from mouse
Synonym(s):
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA′, PECA1, CD31
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
P2B1, monoclonal
Application:
ELISA
flow cytometry
immunocytochemistry
immunofluorescence
immunohistochemistry
immunoprecipitation (IP)
flow cytometry
immunocytochemistry
immunofluorescence
immunohistochemistry
immunoprecipitation (IP)
Species reactivity:
human
Citations:
2
Technique(s):
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Uniprot accession no.:
Product Name
Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free), clone P2B1, 1 mg/mL, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
P2B1, monoclonal
species reactivity
human
concentration
1 mg/mL
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PECAM1(5175)
Related Categories
Analysis Note
Evaluated by Flow Cytometry in human PBMCs.
Flow Cytometry Analysis: 1 µg of this antibody detected PECAM-1 in 1X10E6 human PBMCs.
Flow Cytometry Analysis: 1 µg of this antibody detected PECAM-1 in 1X10E6 human PBMCs.
Application
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in pluripotent cells (Boyd, N. L., et al. (2007). Exp Biol Med. 232(6):833-843.).
Immunoprecipitation Analysis: A representative lot detected PECAM-1 by immunoprecipitation.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).
ELISA Analysis: A representative lot of from an independent laboratory detected PECAM-1 in a panel of CD31 mutant cell lines (Newton, J. P, et al. (1997) J. Biol. Chem., 272: 20555-63.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).
Immunoprecipitation Analysis: A representative lot detected PECAM-1 by immunoprecipitation.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).
ELISA Analysis: A representative lot of from an independent laboratory detected PECAM-1 in a panel of CD31 mutant cell lines (Newton, J. P, et al. (1997) J. Biol. Chem., 272: 20555-63.).
Immunofluorescence Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free) is validated for use in flow cytometry, ICC, IP, IHC, ELISA & immunofluorescence for the detection of PECAM-1.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
PECAM-1 is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.
~80 kDa calculated
Immunogen
Whole cells expressing human PECAM-1.
Other Notes
Replaces: 04-1074, MAB2148
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Laszlo Halasz et al.
International journal of molecular sciences, 25(1) (2024-01-11)
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Nature communications, 8(1), 68-68 (2017-07-14)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB2148-C | 04053252975554 |
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