MAB2226

Anti-Piccolo Antibody, clone 4G3.3

Name: Anti-Piccolo Antibody, clone 4G3.3
Brand: MM

clone 4G3.3, from mouse

Synonym(s):

presynaptic cytomatrix protein, Aczonin

Select a Size

All Photos(2)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-Piccolo Antibody, clone 4G3.3, clone 4G3.3, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

4G3.3, monoclonal

species reactivity

mouse

species reactivity (predicted by homology)

human (based on 82% homology), rat (92% homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgMκ

NCBI accession no.

NP_001104266

UniProt accession no.

Q9Y6V0

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PCLO(27445)
mouse ... Pclo(26875)

Analysis Note

Control
Rat brain lysate, mouse cerebellum

Routinely evaluated by western blot on rat brain lysate.

Application

Immunohistochemistry (Paraffin):
Representative images from a previous lot.
Piccolo staining pattern/morphology on mouse brain tissue/cerebellum. Tissue was pretreated with citrate pH 6.0, antigen retrieval. A previous lot of the antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is localized to the synaptic junction of neurons in the granular layer and Purkinje cells(arrow).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Piccolo Antibody, clone 4G3.3 is validated for use in WB, IH(P) for the detection of Piccolo.

Biochem/physiol Actions

This antibody recognizes piccolo.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

547 kDa

Piccolo (presynaptic cytomatrix protein or PCLO) may act as a scaffolding protein involved in the organization of synaptic active zones. Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins. Among the components of the PCM that orchestrate these events are Bassoon (BSN), RIM (RBBP8), Oboe, and Piccolo (PCLO) [supplied by ENTREZGENE].

Immunogen

GST-tagged recombinant protein

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS and 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c