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MAB2259Z

Sigma-Aldrich

Anti-Integrin β1 Antibody, activated, clone B44, azide free

clone B44, Chemicon®, from mouse

Synonym(s):

Ligand-inducedBindingSite, LIBS, CLIBS, MAB2259

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B44, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

cell culture | mammalian: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002292.3

UniProt accession no.

P55268

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ITGB1(3688)

Related Categories

General description

88 kDa

Application

Immunoprecipitation of detergent-solubilized (1 integrins: 2-4 μg/IP

Western blot: 10-15 μg/mL

Flow Cytometry: 10-15 μg/mL

Immunohistochemistry

Stimulation of (1-mediated adhesion: 10-20 μg/mL.

Optimal working dilutions must be determined by end user.
This Anti-Integrin β1 Antibody, activated, clone B44, azide free is validated for use in FC, IH, IP, FUNC, CULT, WB for the detection of Integrin β1.

Biochem/physiol Actions

Detects a cation- and ligand-induced binding site (LIBS) of integrin beta 1, which has been used in some systems as a reporter of integrin functionality.

Physical form

Format: Purified
Purified immunoglobulin. Liquid PBS, containing no preservatives.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wan-Chun Chen et al.
American journal of physiology. Renal physiology, 307(6), F695-F707 (2014-07-25)
To explore whether matrix stiffness affects cell differentiation, proliferation, and transforming growth factor (TGF)-β1-induced epithelial-mesenchymal transition (EMT) in primary cultures of mouse proximal tubular epithelial cells (mPTECs), we used a soft matrix made from monomeric collagen type I-coated polyacrylamide gel
M Levite et al.
European journal of immunology, 31(12), 3504-3512 (2001-12-18)
Dopamine by itself has not up to now been reported to activate T cell function. We show here that dopamine interacts directly with dopaminergic receptors on normal human T cells and triggers beta1 integrin-mediated T cell adhesion to a major
Tommy Hui et al.
Matrix biology : journal of the International Society for Matrix Biology, 41, 19-25 (2014-12-03)
Osteopontin (OPN) is a ligand for the α4ß1 integrin, but the physiological importance of this binding is not well understood. Here, we have assessed the effect of post-translational modifications on OPN binding to the α4 integrin on cultured human leukocyte
Weisheng Wang et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 41(11), 2723-2732 (2016-06-09)
Estradiol (E2) perfusion rapidly increases the strength of fast excitatory transmission and facilitates long-term potentiation in the hippocampus, two effects likely related to its memory-enhancing properties. Past studies showed that E2's facilitation of transmission involves activation of RhoA signaling leading
Cheng-Te Hsiao et al.
Oncotarget, 8(41), 70653-70668 (2017-10-21)
Directed cell migration is an important step in effective wound healing and requires the dynamic control of the formation of cell-extracellular matrix interactions. Plasma fibronectin is an extracellular matrix glycoprotein present in blood plasma that plays crucial roles in modulating
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Global Trade Item Number

SKUGTIN
MAB2259Z04053252664649

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