MAB2259Z
Anti-Integrin β1 Antibody, activated, clone B44, azide free
clone B44, Chemicon®, from mouse
Synonym(s):
Ligand-inducedBindingSite, LIBS, CLIBS, MAB2259
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
B44, monoclonal
Species reactivity:
human
Application:
FACS, IHC, IP, WB, cell culture
Citations:
16
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
B44, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable, flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ITGB1(3688)
General description
88 kDa
Application
Immunoprecipitation of detergent-solubilized (1 integrins: 2-4 μg/IP
Western blot: 10-15 μg/mL
Flow Cytometry: 10-15 μg/mL
Immunohistochemistry
Stimulation of (1-mediated adhesion: 10-20 μg/mL.
Optimal working dilutions must be determined by end user.
Western blot: 10-15 μg/mL
Flow Cytometry: 10-15 μg/mL
Immunohistochemistry
Stimulation of (1-mediated adhesion: 10-20 μg/mL.
Optimal working dilutions must be determined by end user.
This Anti-Integrin β1 Antibody, activated, clone B44, azide free is validated for use in FC, IH, IP, FUNC, CULT, WB for the detection of Integrin β1.
Biochem/physiol Actions
Detects a cation- and ligand-induced binding site (LIBS) of integrin beta 1, which has been used in some systems as a reporter of integrin functionality.
Physical form
Format: Purified
Purified immunoglobulin. Liquid PBS, containing no preservatives.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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M Levite et al.
European journal of immunology, 31(12), 3504-3512 (2001-12-18)
Dopamine by itself has not up to now been reported to activate T cell function. We show here that dopamine interacts directly with dopaminergic receptors on normal human T cells and triggers beta1 integrin-mediated T cell adhesion to a major
Wan-Chun Chen et al.
American journal of physiology. Renal physiology, 307(6), F695-F707 (2014-07-25)
To explore whether matrix stiffness affects cell differentiation, proliferation, and transforming growth factor (TGF)-β1-induced epithelial-mesenchymal transition (EMT) in primary cultures of mouse proximal tubular epithelial cells (mPTECs), we used a soft matrix made from monomeric collagen type I-coated polyacrylamide gel
Tommy Hui et al.
Matrix biology : journal of the International Society for Matrix Biology, 41, 19-25 (2014-12-03)
Osteopontin (OPN) is a ligand for the α4ß1 integrin, but the physiological importance of this binding is not well understood. Here, we have assessed the effect of post-translational modifications on OPN binding to the α4 integrin on cultured human leukocyte
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB2259Z | 04053252664649 |