Key Documents
MAB308
Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2
ascites fluid, clone 4F10.2, Chemicon®
Synonym(s):
DBH
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
4F10.2, monoclonal
species reactivity
rat, bovine, sheep
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
bovine ... Dbh(280758)
rat ... Dbh(25699)
General description
Dopamine Beta Hydroxylase (DBH), the structural locus encoding DβH enzyme, is a major quantitative trait locus influencing plasma DβH activity, with one single nucleotide polymorphism (SNP) accounting for up to 50% of the variance. Mutations at DBH appear to be responsible for the rare syndrome of DβH deficiency. It functions as the only enzyme involved in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine and epinephrine the only transmitters synthesized inside vesicles. It is expressed in noradrenergic nerve terminals of the central and peripheral nervous systems, as well as in chromaffin cells of the adrenal medulla.
Immunogen
Purified bovine DBH.
Application
Anti-Dopamine β Hydroxylase Antibody, clone 4F10.2 detects level of Dopamine β Hydroxylase & has been published & validated for use in IH, IH(P) & IF.
Indirect immunofluorescence:
1:300-1:500 dilution of a previous lot was used on rat brain tissue.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord
1:300-1:500 dilution of a previous lot was used on rat brain tissue.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord
Biochem/physiol Actions
Dopamine beta hydroxylase (DBH).
Physical form
Ascites mouse monoclonal IgG1 in liquid with 0.1% sodium azide.
Analysis Note
Immunohistochemistry(paraffin): Dopamine β Hydroxylase (cat. # MAB308) staining pattern/morphology in rat spinal cord. Tissue was pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:300, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen predominantly expressed in the dorsal root ganglia (DRG).
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Spinal Cord
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB394-250UG; MAB394-100UG; MAB394-25UG
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB308 | 04053252577741 |
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