Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 5B9.2

The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.

Epitope: near C-terminus, cytoplasmic

Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 5B9.2 detects level of Connexin 45 & has been published & validated for use in IC & WB.

Immunblotting (~46 kDa)

Immunocytochemistry

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Recognizes Human Connexin 45.

SPECIES REACTIVITIES:

Due to sequence homologies, this antibody is also expected to react with canine (100% homology) and mouse (92% homology) Connexin 45. Also confirmed to work on rat C6 cells. Others not tested.

Format: Purified

Liquid at 1 mg/mL in 0.02M PB with 0.25 M NaCl, pH=7.6 containing 0.1% sodium azide.

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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