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Merck

MAB316

Anti-GABA Antibody, clone 5A9

clone 5A9, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
5A9, monoclonal
Technique(s):
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
Application:
ELISA, IHC, WB
Citations:
24
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biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

5A9, monoclonal

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GABRA1(2554)

Immunogen

GABA-coupled to BSA with glutaraldehyde.

Application

Anti-GABA Antibody, clone 5A9 detects level of GABA & has been published & validated for use in ELISA, IH & WB.
Immunohistochemistry on frozen sections if perfusion fixed. Glutaraldehyde must be included in fixative with paraffin sections: 1:200-1:400. Typical fixative might be 4% PFA, 1% glutaraldehyde in 0.1M sodium phosphate buffer, pH 7.3 .

Immunoblotting not recommended due to low molecular wgt: 103Da.

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Biochem/physiol Actions

Specific for GABA and does not cross-react with other amino acids.

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal in PBS, pH 7.4, 1% BSA, and 0.1% sodium azide as a preservative.

Preparation Note

Maintain refrigerated at 2–8°C for up to 6 months.

Analysis Note

Control
Myeloblasts, lymphocytes, erythroblasts

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Neelima Bhargava et al.
In vitro cellular & developmental biology. Animal, 46(8), 685-692 (2010-07-16)
A very small population of choline acetyltransferase (ChAT) immunoreactive cells is observed in all layers of the adult hippocampus. This is the intrinsic source of the hippocampal cholinergic innervation, in addition to the well-established septo-hippocampal cholinergic projection. This study aimed
Naomi Karoubi et al.
Frontiers in neuroanatomy, 10, 106-106 (2016-11-29)
Over recent years, the seven-spot archerfish (Toxotes chatareus) has emerged as a new model for studies in visual and behavioral neuroscience thanks to its unique hunting strategy. Its natural ability to spit at insects outside of water can be used
Alain C Burette et al.
The Journal of comparative neurology, 522(9), 2164-2178 (2014-03-19)
The 53 kDa insulin receptor substrate protein (IRSp53) is highly enriched in the brain. Despite evidence that links mutations of IRSp53 with autism and other neuropsychiatric problems, the functional significance of this protein remains unclear. We used light and electron



Global Trade Item Number

SKUGTIN
MAB31604053252396762