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MAB3199Z

Sigma-Aldrich

Anti-E-Cadherin Antibody, clone 67A4, Azide Free

clone 67A4, Chemicon®, from mouse

Synonym(s):

CD324, MAB3199

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

67A4, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunofluorescence: suitable

isotype

IgG1

NCBI accession no.

NM_004360.2

UniProt accession no.

P12830

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDH1(999)

Related Categories

Immunogen

T-47D breast carcinoma line.

Application

Anti-E-Cadherin Antibody, clone 67A4, Azide Free is an antibody against E-Cadherin for use in FC & IF.
Immunoblotting

FACS Analysis

Functional blocking of E-cadherin

Does not work well for immunoprecipitation

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.

Physical form

Format: Purified
Liquid in 0.02M PBS with NaCl, pH=7.6, with no preservatives. Protein A purified immunoglobulin.

Analysis Note

Tested

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

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