Anti-Beta (β)-Amyloid antibody

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5 is validated for use in WB for the detection of Amyloid Precursor Protein.

Western blot: 10-20 μg/mL

Optimal working dilutions must be determined by the end user.

Westen Blot Protocol for MAB349

1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.

2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.

3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.

4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.

5) Repeat step #3.

6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.

7) Repeat step #3.

8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.

9) Repeat step #3.

Reacts with the natural amyloid precursor protein A4(695).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Epitope: A4(695)

Synthetic peptide Alz 90 [amino acids 511-608 of amyloid precursor protein pre A4(695)].

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Format: Purified

Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.

Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

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