MAB3516

Anti-Heat Shock Protein 70 Antibody, clone W27

Name: Anti-Heat Shock Protein 70 Antibody, clone W27
Brand: MM

clone W27, Chemicon^®, from mouse

Synonym(s):

HSP70

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-Heat Shock Protein 70 Antibody, clone W27, clone W27, Chemicon^®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

W27, monoclonal

species reactivity

bovine, human, monkey

manufacturer/tradename

Chemicon^®

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_002154.3
NM_198431.1

UniProt accession no.

P34932

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... HSPA4(3308)

Analysis Note

Control
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.

Application

Immunoblotting: 0.5-1 μg/mL

Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)

Flow cytometry

Optimal working dilutions must be determined by end user.

This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.

Biochem/physiol Actions

Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.

POSITIVE CONTROL:

HeLa cells, BT474 cells or breast carcinoma.

Immunogen

HSP70 from HeLa cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Advanced ovarian cancer usually spreads to the visceral adipose tissue of the omentum. However, the omental stromal cell-derived molecular determinants that modulate ovarian cancer growth have not been characterized. Here, using next-generation sequencing technology, we identify significantly higher levels of

High dietary taurine reduces apoptosis and atherosclerosis in the left main coronary artery: association with reduced CCAAT/enhancer binding protein homologous protein and total plasma homocysteine but not lipidemia.

Zulli, A; Lau, E; Wijaya, BP; Jin, X; Sutarga, K; Schwartz, GD; Learmont, J; Wookey et al.

Hypertension null

Extracellular vesicles from oviductal isthmus and ampulla stimulate the induced acrosome reaction and signaling events associated with capacitation in bovine spermatozoa.

A Franchi et al.

Journal of cellular biochemistry, 121(4), 2877-2888 (2019-11-07)

Cells can communicate with other neighboring or distant cells through the secretion of extracellular vesicles (EV), composed of a lipid bilayer and bearing surface molecules that allow them to recognize target cells. In this way, EV induce signaling via different

Novel UBQLN2 mutations linked to amyotrophic lateral sclerosis and atypical hereditary spastic paraplegia phenotype through defective HSP70-mediated proteolysis.

Elisa Teyssou et al.

Neurobiology of aging, 58, 239-239 (2017-07-19)

Mutations in UBQLN2 have been associated with rare cases of X-linked juvenile and adult forms of amyotrophic lateral sclerosis (ALS) and ALS linked to frontotemporal dementia (FTD). Here, we report 1 known (c.1489C>T, p.Pro497Ser, P497S) and 3 novel (c.1481C>T, p.Pro494Leu

Characteristics of stem cells from human neuroblastoma cell lines and in tumors.

Jeanette D Walton et al.

Neoplasia (New York, N.Y.), 6(6), 838-845 (2005-02-22)

Cellular heterogeneity is a hallmark of human neuroblastoma tumors and cell lines. Within a single neuroblastoma are cells from distinct neural crest lineages whose relative abundance is significant for prognosis. We postulate that a self-renewing multipotent tumor stem cell, which

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Global Trade Item Number

SKU	GTIN
MAB3516	04053252466021

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