Key Documents
MAB353C3
Anti-Nestin Antibody, clone rat-401, Cy3 conjugate
clone rat-401, from mouse, CY3 conjugate
Synonym(s):
rat401, NES, Nbla00170, FLJ21841
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
rat-401, monoclonal
species reactivity
rat, mouse
should not react with
human
technique(s)
immunocytochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NES(10763)
mouse ... Nes(18008)
rat ... Nes(25491)
General description
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa
Immunogen
Embryonic rat spinal cord
Application
Research Category
Stem Cell Research
Neuroscience
Stem Cell Research
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
This Anti-Nestin Antibody, clone rat-401, Cy3 conjugate is validated for use in ICC for the detection of Nestin.
Biochem/physiol Actions
This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.
Physical form
Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Preparation Note
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Mouse cortical neural stem cells (SCR029)
Mouse cortical neural stem cells (SCR029)
Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB353C3 | 04053252648328 |
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