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Merck

MAB353C3

Anti-Nestin Antibody, clone rat-401, Cy3 conjugate

clone rat-401, from mouse, CY3 conjugate

Synonym(s):

rat401, NES, Nbla00170, FLJ21841

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Nestin Antibody, clone rat-401, Cy3 conjugate, clone rat-401, from mouse, CY3 conjugate

biological source

mouse

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

rat-401, monoclonal

species reactivity

rat, mouse

should not react with

human

technique(s)

immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

NP_037119

UniProt accession no.

P21263

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... NES(10763)
mouse ... Nes(18008)
rat ... Nes(25491)

Analysis Note

Control
Mouse cortical neural stem cells (SCR029)
Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

Application

Research Category
Stem Cell Research

Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers
This Anti-Nestin Antibody, clone rat-401, Cy3 conjugate is validated for use in ICC for the detection of Nestin.

Biochem/physiol Actions

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa

Immunogen

Embryonic rat spinal cord

Physical form

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Preparation Note

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dong Han et al.
Science advances, 8(7), eabe4375-eabe4375 (2022-02-17)
Oct4 collaborates primarily with other transcriptional factors or coregulators to maintain pluripotency. However, how Oct4 exerts its function is still unclear. Here, we show that the Oct4 linker interface mediates competing yet balanced Oct4 protein interactions that are crucial for
Prakasham Rumajogee et al.
eNeuro, 5(5) (2019-02-05)
Cerebral palsy (CP) is a common pediatric neurodevelopmental disorder, frequently resulting in motor and developmental deficits and often accompanied by cognitive impairments. A regular pathobiological hallmark of CP is oligodendrocyte maturation impairment resulting in white matter (WM) injury and reduced

Global Trade Item Number

SKUGTIN
MAB353C304053252648328

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