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Merck

MAB3612

Anti-BubR1 Antibody, clone 8G1

clone 8G1, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-BubR1 Antibody, clone 8G1, clone 8G1, Chemicon®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8G1, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_001211.4

UniProt accession no.

O60566

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... BUB1B(701)

Application

Detect BubR1 with Anti-BubR1 Antibody, clone 8G1 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC.
Western blot using an ECL detection system. Reacts with the ~120 kDa BubR1 protein and higher bands due to hyperphosphorylations.

Immunocytochemistry: 1 μg/mL on tissue culture cells. Suggested fix is 3.5% PBS-buffered paraformaldehyde for 7 minutes. Permeabilization method is 0.2% Triton-X-100 after fixation. Suggested blocking buffer is 10 mM Tris, pH 7.5 with 150 mM NaCl with 0.1% BSA.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes human BubR1.

General description

The mitotic checkpoint prevents the resulting two daughter cells from having unequal numbers of chromosomes. This condition, known as aneuploidy, is present in almost all cancer cells and may be due to malfunctions in the mitotic checkpoint. BubR1 is a mitotic checkpoint kinase and has been implicated in the metaphase checkpoint control in mammalian cells.

Immunogen

Recombinant human BubR1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Subcellular localization of the spindle proteins Aurora A, Mad2, and BUBR1 assessed by immunohistochemistry.
Burum-Auensen, E; De Angelis, PM; Schj?lberg, AR; Kravik, KL; Aure, M; Clausen, OP
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society null
Linda Clijsters et al.
Cell cycle (Georgetown, Tex.), 13(15), 2370-2378 (2014-12-09)
Sister chromatid separation creates a sudden loss of tension on kinetochores, which could, in principle, re-activate the spindle checkpoint in anaphase. This so-called "anaphase problem" is probably avoided by timely inactivation of cyclin B1-Cdk1, which may prevent the spindle tension
David J Wynne et al.
Molecular biology of the cell, 27(22), 3395-3404 (2016-11-05)
The kinetochore is often depicted as having a disk-like architecture in which the outer layer of proteins, which engage microtubules and control checkpoint signaling, are built on a static inner layer directly linked to CENP-A chromatin. Here, applying three-dimensional (3D)
Erik Voets et al.
Biology open, 4(4), 484-495 (2015-03-10)
When cells enter mitosis, the anaphase-promoting complex/cyclosome (APC/C) is activated by phosphorylation and binding of Cdc20. The RXXL destruction box (D-box) of cyclin B1 only binds Cdc20 after release of the spindle checkpoint in metaphase, initiating cyclin B1 ubiquitination upon
Eric C Tauchman et al.
Nature communications, 6, 10036-10036 (2015-12-02)
During mitosis, duplicated sister chromatids attach to microtubules emanating from opposing sides of the bipolar spindle through large protein complexes called kinetochores. In the absence of stable kinetochore-microtubule attachments, a cell surveillance mechanism known as the spindle assembly checkpoint (SAC)
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Global Trade Item Number

SKUGTIN
MAB361204053252723186

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