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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
051007-4A5, monoclonal
Species reactivity:
human
Application:
FACS, ICC, IF, IHC
Citations:
-
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
051007-4A5, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable
input
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
isotype
IgMκ
shipped in
wet ice
target post-translational modification
unmodified
General description
200 kDa
The evolution of our understanding of pluripotent and adult stem cell biology requires specific research tools to permit the characterization, isolation, and expansion of stem cells. HESCA-1, developed in collaboration with Abeome Corporation, is the first in a series of novel, human embyronic stem cell antibodies produced to provide scientists with those needed tools. This novel monoclonal antibody recognizes a newly discovered 200 kDa cell surface protein that has been shown to be expressed in pluripotent human embryonic stem cell (hESC) populations.
Immunogen
BG01v hES cell line
Application
Detect HESCA-1 (Human Embryonic Stem Cell Antigen-1) using this Anti-HESCA-1 Antibody, clone 051007-4A5 validated for use in IC, IH, IF, FC.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
Biochem/physiol Actions
Reactivity to other species has not been determined.
This antibody is positive by immunofluorescence against H1, BG-01, BG01v, and BF02 human embryonic stem (hES) cell lines; however, it is unreactive for differentiated stem cells in teratomas and common feeder lines such as MEF, D-551, and Hs27. Reactivity with other hES lines has not yet been determined.
Physical form
Format: Purified
Liquid in 0.02M PBS, 0.25 NaCl, pH 7.6 with 0.1% Sodium Azide
Preparation Note
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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