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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
AER314, monoclonal
Species reactivity:
mouse, rat, rabbit, bovine, human
Application:
IHC, IP, WB
Citations:
6
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
AER314, monoclonal
species reactivity
mouse, rat, rabbit, bovine, human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ESR1(2099)
Immunogen
Bovine estrogen receptor.
Epitope: a.a. 120-170 of hER-1 alpha
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Use Anti-ER-alpha Antibody, clone AER314 (Mouse Monoclonal Antibody) validated in IP, WB, IHC(P) to detect Estrogen Receptor α also known as ER, ER1, Estradiol Receptor, ER-alpha.
Western blot (human)
Immunoprecipitation (human)
Immunohistochemistry (mouse): faint staining
Immunohistochemistry (human): reactive on paraffin embedded sections after microwaving. *Some cytoplasmic staining may be seen.
Final working dilutions must be determined by end user.
Immunoprecipitation (human)
Immunohistochemistry (mouse): faint staining
Immunohistochemistry (human): reactive on paraffin embedded sections after microwaving. *Some cytoplasmic staining may be seen.
Final working dilutions must be determined by end user.
Biochem/physiol Actions
Estrogen receptor 67kD hormone binding subunit. Epitope localized between aa 120-170.
AFFINITY:
0.8 nM
AFFINITY:
0.8 nM
Physical form
Liquid
Preparation Note
Maintain at -20°C in undiluted aliquots for up to one year. Avoid repeated freeze-thaw cycles.
Other Notes
Replaces: 04-227
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sarah Kimmins et al.
Reproductive biology and endocrinology : RB&E, 1, 38-38 (2003-05-21)
We have shown previously that downregulation of intercaruncular stromal integrin alphavbeta3 in bovine endometrium on day 16 of the estrous cycle coincided with the antibody recognition of estrogen receptors (ER) in the luminal epithelium. In pregnancy, these changes were not
Histopathological, ultrastructural, and immunohistochemical assessment of hippocampus structures of rats exposed to TCDD and high doses of tocopherol and acetylsalicylic acid.
Rosinczuk, J; Dymarek, R; Calkosinski, I
BioMed Research International null
Young coconut juice can accelerate the healing process of cutaneous wounds.
Radenahmad, N; Saleh, F; Sayoh, I; Sawangjaroen, K; Subhadhirasakul, P; Boonyoung et al.
BMC Complementary and Alternative Medicine null