Anti-B23 Antibody, clone 3C9, Ascites Free

Nucleophosmin (UniProt P06748; also known as B23, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin) is encoded by the NPM1 (also known as NPM) gene (Gene ID 4869) in human. Nucleophosmin (NPM) is a nucleolar protein that plays a role in ribosome biogenesis, nucleocytoplasmic transport, centrosome duplication, embryonic development, histone chaperone function, and transcriptional regulation. NPM is phosphorylated by Cdk2-cyclin E on Thr199 which causes the dissociation of NPM from the centrosome to allow duplication during the cell cycle. NPM is phosphorylated by the mitotic Cdk1-cyclin B complex on Thr234/237 during mitosis, resulting in its reassociation with the duplicated centrosomes. The human NPM1 gene transcribes three alternatrively spliced isoforms, isoforms 1&2 contain a nucleolar localization signal at their C-terminal end (W288/290 of isoform 1 and W259/261 of isoform 2), which is missing in the spliced isoform 3. In addition, a nuclear export signal is introduced, rendering the aberrant cytoplasmic location of this spliced isoform (NPMc).

~37 kDa observed.

Daudi nuclear extract.

Anti-B23 Antibody, clone 3C9, Ascites Free is an antibody against B23 for use in Western Blotting, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation.

Western Blotting Analysis: 1.0 µg/mL of this antibody from a representative lot detected B23 in 10 µg of HeLa and Jurkat cell lysates.
Immunocytochemistry Analysis: Representative lots immunostained the nucleoli of paraformaldehyde-fixed, Triton X-100-permeabilized human cells by fluorescent immunocytochemistry (JIn, Y.Q., et al. (2014). Cell Cycle. 13(10):1627-1638; Dergunova, N., et al. (2002). Immunol Lett. 83(1):67-72; Bulycheva, T.I., et al. (2000). Tsitologiia. 42(10):944-954; Bulycheva, T.I., et al. (2000). Klin. Lab. Diagn. (1):37-38).
Immunohistochemistry Analysis: A representative lot immunostained the nucleoli of dopaminergic (DA) neurons in formalin-fixed, paraffin-embedded human and mouse brain sections (Domanskyi, A., et al. (2011). FASEB J. 25(9):2898-2910; Rieker, C., et al. (2011). J. Neurosci. 12;31(2):453-460).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous B23/NPM and associated BRCA2 from COS-7 cell lysate (Wang, H.F., et al. (2011). Cancer Res. 71(1):68-77).
Immunoprecipitation Analysis: A representative lot immunoprecipitated B23 from HeLa cell lysates (Dergunova, N., et al. (2002). Immunol Lett. 83(1):67-72).
Western Blotting Analysis: A representative lot detected the 35-40 kDa B23 doublet bands in HeLa and Ramos cell lysates as well as in HeLa B23 immunoprecipitate obtained with clone 3C9 (Dergunova, N., et al. (2002). Immunol Lett. 83(1):67-72).

Clone 3C9 detected the 37/43 kDa nucleolar protein B23 located in the nucleolar granules in various cells, most intensely in actively proliferating cell cultures (Bulycheva, T.I., et al. (2000). Klin. Lab. Diagn. (1):37-38).

Format: Purified

Purified mouse monoclonal IgMκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected B23 in 10 µg of HEK293 cell lysate.

Concentration: Please refer to lot specific datasheet.