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Merck

MAB4603

Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03

clone 3CSP03, Chemicon®, from mouse

Synonym(s):

CPP32

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03, clone 3CSP03, Chemicon®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3CSP03, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_004346.3
NM_032991.2

UniProt accession no.

P42574

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CASP3(836)

Application

Detect Caspase 3 using this Anti-Caspase 3 Antibody, large subunit & proform, clone 3CSP03 validated for use in IP, WB, IH(P).
Research Category
Apoptosis & Cancer

Metabolism
Research Sub Category
Caspases

Enzymes & Biochemistry
Western blot: 1 μg/ml for 2 hours at RT.

Immunoprecipitation: 2 μg/mg of protein lysate Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes a protein of 32 kDa, identified as the Caspase-3 protein. Highly specific to Caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two subunits of 17 kDa and 12 kDa respectively. Caspase-3 has been implicated in the induction of apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers, including Fas-mediated apoptosis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: large subunit & proform
Recombinant full-length human caspase-3 protein

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-440; 04-1088

Physical form

Format: Purified
Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kaposi's sarcoma herpesvirus microRNAs target caspase 3 and regulate apoptosis.
Suffert, G; Malterer, G; Hausser, J; Viiliainen, J; Fender, A; Contrant, M; Ivacevic et al.
PLoS Pathogens null

Global Trade Item Number

SKUGTIN
MAB460304053252398551

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