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Merck

MAB463

Anti-Estrogen Receptor Antibody, clone B10

ascites fluid, clone B10, Chemicon®

Synonym(s):

ER

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Estrogen Receptor Antibody, clone B10, ascites fluid, clone B10, Chemicon®

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

B10, monoclonal

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2a

NCBI accession no.

NP_000116.2

UniProt accession no.

P03372

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... HSPG2(3339)

Application

Immunohistochemistry (frozen sections, microwave oven treated paraffin sections) at 1:1,000-1:2,000

Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000

ELISA at 1:1,000-1:2,000

Western blot analysis at 1:1,000-1:2,000

Immunoprecipitation at 1:1-1:10

Gel super shifts at 1:1-1:10

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-Estrogen Receptor Antibody, clone B10 (Mouse Monoclonal Antibody) validated in ELISA, WB, IHC(P) to detect Estrogen Receptor also known as ER.

Biochem/physiol Actions

The peptide used as the immunogen for this product correspond to the amino-acid sequence 151-165 from human ER alpha receptor. By IP, western, gel retardation and ELISA assays clone B10 do not react with other nuclear receptors.

SPECIES REACTIVITIES:

Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: a.a. 151-165

Physical form

Ascites. Liquid, does not contain any preservative.

Preparation Note

Maintain at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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P Bhat-Nakshatri et al.
British journal of cancer, 90(4), 853-859 (2004-02-19)
Oestrogen receptor alpha (ERalpha) is an oestrogen-activated transcription factor, which regulates proliferation and differentiation of mammary epithelial cells by activating or repressing gene expression. ERalpha is a critical prognostic indicator and a therapeutic target for breast cancer. Patients with tumours
Maie Dawoud Al-Bader et al.
Reproductive sciences (Thousand Oaks, Calif.), 22(4), 484-494 (2014-09-14)
Metastasis-associated protein 1 (MTA1) and its short form (MTA1s) regulate the function of estrogen receptors (ERs). Estrogens, via ERs, affect placental growth and fetal development, a process that may involve MTA1 signaling. Expression of MTA1, MTA1s, ERα, and ERβ genes

Global Trade Item Number

SKUGTIN
MAB46304053252666230

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