MAB5302
Anti-Acetylcholine Antibody
Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Acetylcholine Antibody, Chemicon®, from mouse
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
monoclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
isotype
IgG2a
shipped in
dry ice
Quality Level
Gene Information
rat ... Ache(83817)
Application
Immunohistochemistry: 1:250-1:2,500 using free floating sections by the PAP technique on rat cholinergic areas. Tissues should be fixed with a mixture of nitrobenzyl alcohol and gluteraldehyde (Chagnaud, 1989). Gluteraldehyde is required for staining; not recommended for formalin or paraformaldehyde fixations without gluteraldehyde.
ELISA: 1:1,000-1:5,000
Immunocytochemistry: 1:1,000-1:5,000
Western Blot: 1:1,000-1:2,000
Optimal working dilutions must be determined by end user.
ELISA: 1:1,000-1:5,000
Immunocytochemistry: 1:1,000-1:5,000
Western Blot: 1:1,000-1:2,000
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
This Anti-Acetylcholine Antibody is validated for use in IH for the detection of Acetylcholine.
Biochem/physiol Actions
Acetylcholine. By immunohistochemistry on rat basal forbrain and spinal chord sections, the antibody specifically stains nerve cell bodies and fibres (Chagnaud, 1989).
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
Choline-GA-BSA 1
Choline-AA-BSA 1/536
Choline-SA-BSA 1/2,500
Farnesol-BSA 1/>50,000
Retinoic acid-BSA 1/>50,000
Myristic acid-BSA 1/>50,000
Acetylcholine 1/>50,000
Choline 1/>50,000
Abbreviations:
(GA) Glutaric anhydride
(AA) Adipic acid
(SA) Succinic acid
(BSA) Bovine Serum Albumin
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity
Choline-GA-BSA 1
Choline-AA-BSA 1/536
Choline-SA-BSA 1/2,500
Farnesol-BSA 1/>50,000
Retinoic acid-BSA 1/>50,000
Myristic acid-BSA 1/>50,000
Acetylcholine 1/>50,000
Choline 1/>50,000
Abbreviations:
(GA) Glutaric anhydride
(AA) Adipic acid
(SA) Succinic acid
(BSA) Bovine Serum Albumin
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Choline-Glutaric anhydride-BSA.
Physical form
Format: Purified
Liquid containing 0.05% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Therapeutic Effect of Yi-Chi-Tsung-Ming-Tang on Amyloid ?-Induced Alzheimer's Disease-Like Phenotype via an Increase of Acetylcholine and Decrease of Amyloid ?.
Yeh, CH; Hsieh, MT; Hsueh, CM; Wu, CR; Huang, YC; Liao, JW; Chow, KC
Evidence-Based Complementary and Alternative Medicine : ECAM null
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