Key Documents

COA/COQ

MAB5432-C

Anti-CREB Antibody, clone LB9, Ascites Free

Name: Anti-CREB Antibody, clone LB9, Ascites Free
Brand: MM

clone LB9, from mouse

Synonym(s):

Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1, CREB-1

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LB9, monoclonal

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_604391

UniProt accession no.

P16220

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CREB1(1385)

General description

Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation on by various kinases, including PKA and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.

~37-43 kDa observed. 36.69 kDa (isoform CREB-A) calculated.

Application

Anti-CREB Antibody, clone LB9, Ascites Free is an antibody against CREB for use in Western Blotting, Immunohistochemistry (Paraffin).

Western Blotting Analysis: 1.0 µg/mL of this antibody from a representative lot detected CREB in 10 µg of NIH/3T3 and PC12 cell lysate.
Immunohistochemistry Analysis: An 1:50-250 dilution of this antibody from a representative lot detected CREB in human liver, colon, and tonsil tissue sections.
Western Blotting Analysis: Representative lots detected CREB in primary mouse astrocytes and mouse dorsal hippocampal tissue lysates (Orr, A.G., et al. (2015). Nat. Neurosci. 18(3):423-434; Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).
Western Blotting Analysis: Representative lots detected CREB in primary human fibroblast and SH-SY5Y human neuroblastoma cell lysates (Maj, M., et al. (2011). Mitochondrion. 11(2):264-272; Fukuda, Y., et al. (2010). Mol. Cell Neurosci. 45(3):226-233).
Immunohistochemistry Analysis: A representative lot immunostained total CREB in dorsal hippocampal tissue sections from paraformaldehyde-perfused mice (Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).

Biochem/physiol Actions

Clone LB9 reactivity towarad spliced isoform CREB-B and htCREB (isoform 3) has not been determined.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CREB in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)