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Merck

MAB8560

Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4

ascites fluid, clone 583-G8-G2-A4, Chemicon®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4, ascites fluid, clone 583-G8-G2-A4, Chemicon®

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

583-G8-G2-A4, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable

isotype

IgG1

shipped in

wet ice

Quality Level

300

Application

Detect Poliovirus 1 using this Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4 validated for use in IF.
IFA at 1:100-1:400.

Optimal working dilutions must be determined by end user.

SUGGESTED IFA

PROTOCOL:

Substrate slides:

1. Scrape and spot cells onto microscope slide wells.

2. Air Dry.

3. Fix in cold acetone for 10 minutes at +4°C.

Stain:

1. Add 15 μL diluted polio antibody.

2. Incubate at +37°C for 30 minutes in a humid chamber.

3. Rinse in PBS for 5 minutes; repeat with fresh PBS.

4. Add 15 μL diluted labeled antibody.

5. Repeat steps 2 and 3

6. Mount with buffered glycerol.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Biochem/physiol Actions

Reacts with the Poliovirus 1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Store at -20°C for up to 12 months in convenient aliquots. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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William C Weldon et al.
Methods in molecular biology (Clifton, N.J.), 1387, 145-176 (2016-03-18)
Testing for neutralizing antibodies against polioviruses has been an established gold standard for assessing individual protection from disease, population immunity, vaccine efficacy studies, and other vaccine clinical trials. Detecting poliovirus specific IgM and IgA in sera and mucosal specimens has
Mohsan Saeed et al.
PLoS pathogens, 16(9), e1008927-e1008927 (2020-10-01)
Viruses cleave cellular proteins to remodel the host proteome. The study of these cleavages has revealed mechanisms of immune evasion, resource exploitation, and pathogenesis. However, the full extent of virus-induced proteolysis in infected cells is unknown, mainly because until recently

Global Trade Item Number

SKUGTIN
MAB856004053252501968

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