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Merck

MAB8566

Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1, and 613-F1-B5-E5

ascites fluid, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1, and 613-F1-B5-E5, ascites fluid, Chemicon®, from mouse

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

583-G8-G2-A4, monoclonal
591-B1-H7-D1, monoclonal
613-F1-B5-E5, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable

isotype

IgG

shipped in

dry ice

Quality Level

300

Application

Detect Poliovirus Blend using this Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1 & 613-F1-B5-E5 validated for use in IF.
IFA at 1:100-1:400.

Dilute with buffer pH 7.5-8.0 to desired working volume.

Final working dilutions must be determined by end user.

SUGGESTED IFA PROTOCOL:

Substrate slides:

1. Scrape and spot cells onto microscope slide wells.

2. Air dry.

3. Fix in cold acetone for 10 minutes at +4°C.

Stain:

1. Add 15 μl diluted polio antibody.

2. Incubate at +37°C for 30 minutes in a humid chamber.

3. Rinse in PBS for 5 minutes; repeat with fresh PBS.

4. Add 15 μl diluted labeled antibody.

5. Repeat steps 2 and 3.

6. Mount with buffered glycerol.

Biochem/physiol Actions

Reacts with the Poliovirus 1, 2 and 3.

Preparation Note

Maintain at -20°C in convenient undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Johanna Marsian et al.
Nature communications, 8(1), 245-245 (2017-08-16)
Poliovirus (PV) is the causative agent of poliomyelitis, a crippling human disease known since antiquity. PV occurs in two distinct antigenic forms, D and C, of which only the D form elicits a robust neutralizing response. Developing a synthetically produced
Mohammad W Bahar et al.
NPJ vaccines, 6(1), 5-5 (2021-01-10)
Global vaccination programs using live-attenuated oral and inactivated polio vaccine (OPV and IPV) have almost eradicated poliovirus (PV) but these vaccines or their production pose significant risk in a polio-free world. Recombinant PV virus-like particles (VLPs), lacking the viral genome

Global Trade Item Number

SKUGTIN
MAB856604053252502446

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