MABC110

Anti-WIPI-2, clone 1B8.1 Antibody

Name: Anti-WIPI-2, clone 1B8.1 Antibody
Brand: MM

clone 1B8.1, from mouse

Synonym(s):

WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2

Select a Size

All Photos(2)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-WIPI-2, clone 1B8.1 Antibody, clone 1B8.1, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1B8.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001028690

UniProt accession no.

Q9Y4P8

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... WIPI2(26100)

Immunogen

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of human WIPI-2.

Analysis Note

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected WIPI-2 in 10 µg of human testis tissue lysate.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected WIPI-2 in human pancreas tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-WIPI-2, clone 1B8.1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of WIPI-2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

WIPI-2, also known as Atg18, is involved in autophagy by regulating the assembly of mulitprotein complexes by presenting a 7-bladed propeller structure for interactions with phospholipids. Atg18 is a mammalian effector of PtdIns3P and is ubiquitously expressed in a variety of cell lines. Studies show that Atg18 is recruited to early autophagosomal structural formation along with Atg16 and ULK1, and Atg18 is required for the formation of LC3-positive autophagosomes.

~49 kDa observed. Uncharacterized band(s) may appear in some lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Not finding the right product?

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c