Anti-LIMD2 Antibody, clone 18.30

LIM domain-containing protein 2 (UniProt Q9BT23) is encoded by the LIMD2 (also known as SB143) gene (Gene ID 80774) in human. The LIM domain is organized as a tandem zinc-finger structure that functions as a modular protein-binding interface and LIM domain-containing proteins play diverse roles in gene expression, cyto-architecture, cell adhesion, cell motility, and signal transduction. Human LIM domain-only (LMO) proteins are involved in many normal developmental processes, as well as in the onset and progression of several cancers, including T-cell leukemia, breast cancer, and neuroblastoma. LIMD2 is a LMO protein that is highly expressed in papillary thyroid carcinoma (PTC) lymph node metastasis (LNM), but absent from the primary tumor or normal thyroid tissue in matched PTC patient samples. LIMD2 regulates cell motility and affects tumor progression via its role in the integrin-linked kinase (ILK) pathway. LIMD2 is reported to activate ILK activity via direct binding to ILK kinase domain near the active site, LIMD2-knockdown in cancer cells results in reduced cell migration. Likewise, epitopic LIMD2 expression promotes the migration of ILK-expressing fibroblasts, but not fibroblasts lacking ILK.

~17 kDa observed. 14.07 kDa calculated. Uncharacterized band(s) may appear in some lysates.

GST-tagged recombinant full-length human LIMD2.

Anti-LIMD2 Antibody, clone 18.30 is an antibody against LIMD2 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry.

Research Category
Apoptosis & Cancer

Research Sub Category
Adhesion (CAMs)

Western Blotting Analysis: 0.25 µg/mL from a representative lot detected LIMD2 in human U2OS osteosarcoma cell lysate.
Immunocytochemistry Analysis: A representative lot detected exogenously expressed LIMD2 protein constructs in formaldehyde-fixed, Triton X-100-permeabilized cells transfected with epitopically tagged LIMD2 (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Immunohistochemistry Analysis: A representative lot detected LIMD2 immunoreactivity in formalin-fixed, paraffin-embedded papillary thyroid cancer and bladder tumor sections (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).
Western Blotting Analysis: A representative lot detected endogenous LIMD2 in HEK293 and U2OS cell lysates, as well as epitopically tagged LIMD2, but not LIMD1 or AJUBA recombinant protein constructs (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).

Clone 18.30 detected endogenous LIMD2 in human (HEK293, U2OS, and TPC-1) cell lysates, as well as recombinant human LIMD2, but not LIMD1 or AJUBA protein (Peng, H., et al. (2014). Cancer Res. 74(5):1390-1403).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected LIMD2 in human HEK293 kidney embryonic cell lysate.

Concentration: Please refer to lot specific datasheet.

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