MABC286

Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2

Name: Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2
Brand: MM

clone 7D1.2, from mouse

Synonym(s):

Tankyrase-1, TANK1, ADP-ribosyltransferase diphtheria toxin-like 5, ARTD5, Poly [ADP-ribose] polymerase 5A, TNKS-1, TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyrase I

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2, clone 7D1.2, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7D1.2, monoclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG3κ

NCBI accession no.

NP_003738

UniProt accession no.

O95271

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TNKS(8658)

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Tankyrase-1/TNKS in 10 µg of HeLa cell lysate.

Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Tankyrase-1/TNKS in Hela, A431, and N1H/3T3 cells.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Tankyrase-1/TNKS in neurons and glial cells in human cerebral cortex tissue, Purkinje, basket and granule cells in human cerebellum tissue, and neurons in human thalamus tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2 is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of Tankyrase-1/TNKS.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tankyrase-1/TNKS is a critical enzyme in Wnt signaling pathway. Tankyrase-1/TNKS is a poly-ADP-ribosyltransferase which is a key mediator of PARsylation: Becoming ADP-ribosylated. PARsylation is important in Wnt signaling because ADP-ribosylation of AXIN1 and AXIN2, two key components of the beta catenin destruction complex, is necessary for destruction via RNF146 (an ubiquitin lyase) and ubiquitination and subsequent destruction of beta catenin. Tankyrase-1/TNKS also mediates PARsylation of TERF1 and thus is important in controlling telomere length, and Tankyrase-1/TNKS’s PARsylation of other proteins makes Tankyrase-1/TNKS important in vesicle trafficking and subcellular protein distribution as well. Tankyrase-1/TNKS is widely expressed, and is found mostly cytoplasmically but can be associated with the nucleus and spindles and Golgi complexes as well.

~142 kDa observed.
Uncharacterized bands may be observed in some lysates.

Immunogen

GST-tagged recombinant protein corresponding to Human Tankyrase-1/TNKS.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c