Key Documents
conjugate
unconjugated
Quality Level
antibody form
purified antibody
clone
2F11.1
monoclonal
purified by
using protein G
species reactivity
human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
UniProt accession no.
target post-translational modification
unmodified
General description
GST-tagged recombinant protein corresponding to human PELP1.
~160 kDa observed. PELP1/MNAR has a predicted molecular weight of 120 kDa and an isoelectric point of 4.30, but because of the overall negative charge and excessive amount of prolines, the protein migrates on SDS-PAGE as a 160-kDa protein (Rajhans, R., and Vadlamudi, R.K. (2006). Clin. Exp. Metastasis. 23(1):1-7).
Application
Immunocytochemistry Analysis: 5 µg/mL from a representative lot detected PELP1 in HeLa and A431 cells.
Physical form
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PELP1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PELP1 in 10 µg of HeLa cell lysate.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABC303 | 04055977169836 |
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