MABC587
Anti-Nucleolin Antibody, clone 364-5-10-5
clone 364-5-10-5, from mouse
Synonym(s):
Nucleolin, Protein C23
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About This Item
eCl@ss:
32160702
UNSPSC Code:
12352203
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
364-5-10-5, monoclonal
species reactivity
human, mouse
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG1λ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NCL(4691)
General description
Nucleolin, also known as C23, is the major nucleolar protein of exponentially growing eukaryotic cells. It is a ubiquitous conserved multi-domain phosphoprotein found in all vertebrate species. Nucleolin contains a specific bipartite nuclear localization signal sequence and three different structural domains. Nucleolin is a multifunctional protein involved in most steps of ribosome biogenesis. Nucleolin has been implicated in chromatin structure, rDNA transcription, rRNA maturation, ribosome assembly, cytokinesis, nucleogenesis, cell proliferation and growth, and
nucleo-cytoplasmic transport. Nucleolin also exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation, and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2, and protein kinase C-ζ.
nucleo-cytoplasmic transport. Nucleolin also exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation, and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2, and protein kinase C-ζ.
~100 kDa calculated. This protein has many residues that are post transitionally modified (phosphorylation) and can run higher than the calculated molecular weight of 76 kDa in some lysates or have bands at both 76 and ~100-110 kDa (Qiu, J., et al. (1999) Virology. 257(2):373–382.)
Immunogen
Corresponding to human Nucleolin.
Application
This Anti-Nucleolin Antibody, clone 364-5-10-5 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) and Immunocytochemistry and Flow Cytometry for the detection of Nucleolin.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Nucleolin in 10 µg of NIH/3T3 cell lysate.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Nucleolin in human breast cancer and human kidney tissue.
Immunohistochemistry Analysis: A 1:10 dilution from a representative lot detected Nucleolin in ovarian cancer metastasis-infiltrated human lymph node tissue.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Nucleolin in A431 and HEK293 cells.
DyLight® is a registered trademark of Thermo Fisher Scientific.
Flow Cytometry Analysis: A representative lot detected Nucleolin in NK-92MI cells gated and ungated for CD56.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Nucleolin in human breast cancer and human kidney tissue.
Immunohistochemistry Analysis: A 1:10 dilution from a representative lot detected Nucleolin in ovarian cancer metastasis-infiltrated human lymph node tissue.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Nucleolin in A431 and HEK293 cells.
DyLight® is a registered trademark of Thermo Fisher Scientific.
Flow Cytometry Analysis: A representative lot detected Nucleolin in NK-92MI cells gated and ungated for CD56.
Physical form
Format: Purified
Purified mouse monoclonal IgG1λ in buffer containing PBS without preservatives.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Nucleolin in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Nucleolin in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: MABE466
Legal Information
DyLight is a registered trademark of Pierce Biotechnology, Inc.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ravinder Kour et al.
RNA (New York, N.Y.), 25(10), 1377-1392 (2019-07-17)
Eukaryotic ribosomal protein L13a is a member of the conserved universal ribosomal uL13 protein family. Structurally, L13a is distinguished from its prokaryotic counterparts by the presence of an ∼55 amino acid-long carboxy-terminal α-helical extension. The importance of these evolved residues