Key Documents

COA/COQ

MABC965

Anti-Prohibitin-1 (PHB1), clone 1G5C1 Antibody

Name: Anti-Prohibitin-1 (PHB1), clone 1G5C1 Antibody
Brand: MM

clone 1G5C1, from rat

Synonym(s):

Prohibitin

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rat

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1G5C1, monoclonal

species reactivity

human, mouse, rat

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001268425

UniProt accession no.

P35232

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PHB(5245)

General description

Prohibitin is an inhibitor of DNA synthesis that is widely expressed across various tissues. Prohibitin influences proliferation, and may also have a role in regulating mitochondrial respiration activity and in aging. Expression levels of prohibitin in fibroblasts have been found to decrease heterogeneously as cellular aging occurs.

~30 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human Prohibitin-1 (PHB1).

Application

Anti-Prohibitin-1 (PHB1), clone 1G5C1 | MABC965 is an antibody against Prohibitin-1 for use in Western Blotting, Immunohistochemistry.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Prohibitin-1 (PHB1) in 10 µg of NIH/3T3 and HeLa cell lysate.
Immunohistochemistry Analysis: A representative lot detected Prohibitin-1 (PHB1) in rat liver tissue (Kakehashi, A., et al. (2011). Toxicological Sciences. 119(1):61-72).

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in rat liver tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Prohibitin-1 (PHB1) in 10 µg of rat liver tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)