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MABC987

Sigma-Aldrich

Anti-Semaphorin 4D/CD100 Antibody, clone BMA-12

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

conjugate

unconjugated

Quality Level

300

antibody form

purified antibody

clone

BMA-12
monoclonal

purified by

using protein G

species reactivity

mouse, rat

concentration

(Please refer to lot specific datasheet.)

technique(s)

activity assay: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

UniProt accession no.

O09126

target post-translational modification

unmodified

General description

Recombinant protein corresponding to mouse Semaphorin 4D/CD100.
~110 kDa observed. Uncharacterized band(s) may appear at 20-30 kDa in some lysates.

Application

Flow Cytometry Analysis: 1.0 µg of this antibody from a representative lot detected Semaphorin 4D/CD100 in 1X10E6 mouse splenocyte.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Semaphorin 4D/CD100 in 2B4 cells (Wang, X., et al. (2001). BLOOD. 97(11):3498-3504).
Activity Assay Analysis: A representative lot detected Semaphorin 4D/CD100 in 2B4 cells (Wang, X., et al. (2001). BLOOD. 97(11):3498-3504).
Immunohistochemistry Analysis: A representative lot detected Semaphorin 4D/CD100 in mouse kidney sections (Li, M., et al. (2009). Immunology. 128:114-122).
Western Blotting Analysis: A representative lot detected Semaphorin 4D/CD100 in mCD100-FC lysate (Kumanogoh, A., et al. (2000). Immunity. 13:621-631).
Flow Cytometry Analysis: A representative lot detected Semaphorin 4D/CD100 in mouse splenocytes (Kumanogoh, A., et al. (2000). Immunity. 13:621-631).

Physical form

Purified rat monoclonal IgG2a in buffer containing PBS without preservatives.

Preparation Note

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting of msCD100-Fc recombinant protein.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Semaphorin 4D/CD100 in 10 µg of msCD100-Fc recombinant protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MABC98704055977161557

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