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Merck

MABD398

Anti-DAX-1, clone 2F4 Antibody

ascites fluid, clone 2F4, from mouse

Synonym(s):

Dax1, Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1, AHC

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2F4, monoclonal
Application:
ICC, IHC, WB
Citations:
6

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2F4, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_000466

UniProt accession no.

P51843

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... NR0B1(190)

General description

DAX-1 is an orphan nuclear receptor and is required for the normal development of the hypothalamic-pituitary-adrenal-gonadal axis formation. Defects in DAX-1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC); also known as X-linked Addison disease (AHX). DAX-1 also has a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency by acting as a negative regulator of Oct3/4 in ES cells.
~ 52 kDa observed

Immunogen

Linear peptide corresponding to Human DAX-1 (amino acids 135-166).

Application

Research Sub Category
Pluripotent & Early Differentiation
Anti-DAX-1, clone 2F4, has been shown to work in Immunocytochemical (Lehmann et al., 2009) and Immunohistochemical (Ehrlund et al., 2013) analysis.
Research Category
Stem Cell Research
This Anti-DAX-1, clone 2F4 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of DAX-1.

Physical form

Mouse monoclonal IgG1κ Ascites with 0.05% sodium azide.
Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blot in a Dax-1 overexpressing HEK293 transfected cell line. Western Blot Analysis: A 1:1000 dilution of this antibody detected DAX-1 in a DAX-1 overexpressing HEK293 transfected cell line.

Other Notes

Replaces: 04-445

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yoko Hiyama et al.
Cells, 11(19) (2022-10-15)
We previously used microarrays to show that high expression of DHRS3, NROB1, and CYP26A1 predicts favorable NB outcomes. Here, we investigated whether expression of these genes was associated with suppression of NB cell (SK-N-SH, NB12, and TGW) growth. We assessed
Dax1 modulates ERI?-dependent hypothalamic estrogen sensing in female mice.
Ramos-Pittol, et al.
Nature Communications, 14, 3076-3076 (2023)

Global Trade Item Number

SKUGTIN
MABD39804053252986512

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