Anti-PRMT9 Antibody, clone 128-29-1

Putative protein arginine N-methyltransferase 9 (UniProt Q6P2P2; also known as Putative protein arginine N-methyltransferase 10) is encoded by the PRMT9 (also known as PRMT10) gene (Gene ID 90826) in human. Mammalian protein arginine methyltransferases (PRMTs) catalyze the methylation of arginine residues on histone and non-histone proteins. PRMTs are divided into three types based on their mode of activity. Type I PRMTs (PRMT1–4, PRMT6, and PRMT8) transfer up to two methyl groups on the same terminal guanidino nitrogen of arginine to form ω-NG-monomethylarginine (MMA) and ω-NG,NG-asymmetric dimethylarginine (ADMA) residues. Type II PRMTs (PRMT5 and PRMT9) transfer methyl groups on different terminal guanidino nitrogens to form ΜΜΑ and ω-NG,N′G-symmetric dimethylarginine (SDMA) residues, while type III PRMT7 transfers only one single methyl group to form MMA residues. PRMT9 is involved in alternative splicing regulation by modulating spliceosomal small nuclear ribonucleoprotein (snRNP) maturation in the cytoplasm. PRMT9 complexes with the splicing factors SF3B2 (SAP145) and SF3B4 (SAP49) and mediates SF3B2 Arg508 modification via MMA and SDMA. Similar to PRMT7, but unlike other PRMTs, PRMT9 contains a duplicated methyltransferase domain (a.a. 137-446 and 530-845), with the N-terminal MTase acting as a funtional AdoMet-binding domain and the C-terminal MTase domain serving a pseudodimer function, mimicking the homodimer formation of other PRMTs needed for subtrate processing. In addition, PRMT9 contains three tetratricopeptide repeats (TPR1-3; a.a. 25-58, 67-100, 101-134) known to mediate protein-protein interactions.

~94 kDa observed. 94.50 kDa (isoform 1) and 81.86 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Epitope: First MTase domain.

GST-tagged recombinant protein corresponding to the N-terminal half of human PRMT9.

Anti-PRMT9 Antibody, clone 128-29-1 is an antibody against PRMT9 for use in Western Blotting, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
General Post-translation Modification

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected PRMT9 in 20 µg of HeLa cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PRMT9 in human kidney, testis, and cerebellum tissue sections.
Immunocytochemistry Analysis: A representative lot detected a predominant cyotoplasmic PRMT9 localization by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed HeLa cells (Yang, Y., et al. (2015). Nat. Commun. 6:6428).
Immunoprecipitation Analysis: A representative lot immunoprecipitated PRMT9 from HeLa cell lysates (Yang, Y., et al. (2015). Nat. Commun. 6:6428).
Western Blotting Analysis: A representative lot detected GST fusion protein containing human PRMT9 a.a. 401-550 recombinant fragment, but not PRMT9 a.a. 1-199, 200-400, or 551-895 GST fusion (Yang, Y., et al. (2015). Nat. Commun. 6:6428).
Western Blotting Analysis: A representative lot detected human, but not mouse, PRMT9 using lysates from various human and mouse cell lines (Yang, Y., et al. (2015). Nat. Commun. 6:6428).

Clone detected GST fusion containing recombinant human PRMT9 fragment a.a. 401-550, as well as endogenous human, but not mouse, PRMT9 in cell lysates (Yang, Y., et al. (2015). Nat. Commun. 6:6428). Epitope region is present in both spliced isoforms of human PRMT9 reported by UniProt (Q6P2P2).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PRMT9 in 20 µg of A431 cell lysate.

Concentration: Please refer to lot specific datasheet.

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