Key Documents

COA/COQ

MABE1114

Anti-CENP-C Antibody, clone AL61A

Name: Anti-CENP-C Antibody, clone AL61A
Brand: MM

clone AL61A, from mouse

Synonym(s):

Centromere protein C, CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AL61A, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

NCBI accession no.

NP_001803

UniProt accession no.

Q03188

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CENPC(1060)

General description

106.8 kDa (isoform 1) and 61.54 kDa (isoform 2) calculated.

Centromere protein C (UniProt Q03188; also known as CENP-C, CENP-C 1, Centromere autoantigen C, Centromere protein C 1, Interphase centromere complex protein 7) is encoded by the CENPC (also known as CENPC1, ICEN7) gene (Gene ID 1060) in human. Kinetochores assemble on centromeres to bind spindle microtubules and mediate chromosome segregation during cell division. CENP-C is a subunit of constitutive centromere-associated network (CCAN) that acts as the centromere-kinetochore interface. CCAN is a large complex composed of at least 16 different centromeric proteins (CENPs). CENP-C plays an important role in kinetochore assembly downstream of CENP-A. The PEST domain in the N-terminal half of CENP-C interacts directly with the CCAN subcomplex CENP-HIKM (CENP-H, CENP-K, CENP-I, and CENP-M) and CENP-C depletion is shown to casue mislocalization of CENP-T and the CENP-HIKM complex subunits in HeLa cells.

Immunogen

Epitope: N-terminal.

GST-tagged recombinant human CENP-C N-terminal fragment.

Application

Anti-CENP-C Antibody, clone AL61A is an antibody against CENP-C for use in Immunocytochemistry, Immunoprecipitation.

Immunoprecipitation Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant immunoprecipitated CENP-C from HeLa nuclear extract (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
Immunocytochemistry Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant detected nuclear CENP-C puncta by fluorescent immunocytochemistry staining of permeabilized HeLa and Jurkat cells (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).
Immunocytochemistry Analysis: Prior to antibody purification, clone AL61A hybridoma culture supernatant immunostained CENP-C-transfected, but not ZBTB32-transfected, HEK293T cells (Courtesy of Dr. Giovanna Roncador, Spanish National Cancer Research Centre (CNIO), Madrid, Spain).

Biochem/physiol Actions

Expected to react with both spliced isoforms of human CENP-C reported by UniProt (Q03188).

Physical form

Format: Purified

Analysis Note

Evaluated by Immunocytochemistry in Jurkat cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected CENP-C in Jurkat cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)