Anti-TET3 Antibody, clone 23B9

180.4 calculated.

Methylcytosine dioxygenase TET3 (EC 1.14.11.n2; UniProt Q8BG87) is encoded by the Tet3 gene (Gene ID 194388) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.

Epitope: Region between DNA-binding domain and 2-oxoglutarate-binding domain.

His-tagged recombinant internal fragment from the region between the DNA-binding domain and 2-oxoglutarate-binding domain of mouse TET3.

Anti-TET3 Antibody, clone 23B9 is an antibody against TET3 for use in Immunocytochemistry, Immunoprecipitation, ELISA.

Immunoprecipitation Analysis: A representative lot immunoprecipitated GFP-tagged TET3 protein from lysates of HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Analysis: Clone 23B9 hybridoma culture supernatant was verified for its immunoreactivity against His-tagged murine TET3 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Developmental Signaling

Clone 23B9 epitope lies within the region between DNA-binding and 2-oxoglutarate-binding domains of murine TET3 sequence (UniProt Q8BG87-1). Clone 23B9 epitope region is not present in spliced isoform 2 (UniProt Q8BG87-2).

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry in mouse ES cells.

Immunocytochemistry Analysis: 1.0 µg/mL of this antibody detected TET3 in mouse ES cells.

Concentration: Please refer to lot specific datasheet.

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