MABE1133
Anti-TET3 Antibody, clone 23B9
clone 23B9, from rat
Synonym(s):
Methylcytosine dioxygenase TET3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rat
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
23B9, monoclonal
species reactivity
mouse
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
General description
180.4 calculated.
Methylcytosine dioxygenase TET3 (EC 1.14.11.n2; UniProt Q8BG87) is encoded by the Tet3 gene (Gene ID 194388) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.
Immunogen
Epitope: Region between DNA-binding domain and 2-oxoglutarate-binding domain.
His-tagged recombinant internal fragment from the region between the DNA-binding domain and 2-oxoglutarate-binding domain of mouse TET3.
Application
Anti-TET3 Antibody, clone 23B9 is an antibody against TET3 for use in Immunocytochemistry, Immunoprecipitation, ELISA.
Immunoprecipitation Analysis: A representative lot immunoprecipitated GFP-tagged TET3 protein from lysates of HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Analysis: Clone 23B9 hybridoma culture supernatant was verified for its immunoreactivity against His-tagged murine TET3 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Analysis: Clone 23B9 hybridoma culture supernatant was verified for its immunoreactivity against His-tagged murine TET3 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Developmental Signaling
Developmental Signaling
Biochem/physiol Actions
Clone 23B9 epitope lies within the region between DNA-binding and 2-oxoglutarate-binding domains of murine TET3 sequence (UniProt Q8BG87-1). Clone 23B9 epitope region is not present in spliced isoform 2 (UniProt Q8BG87-2).
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in mouse ES cells.
Immunocytochemistry Analysis: 1.0 µg/mL of this antibody detected TET3 in mouse ES cells.
Immunocytochemistry Analysis: 1.0 µg/mL of this antibody detected TET3 in mouse ES cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Hongjie Pan et al.
Cell death & disease, 11(2), 142-142 (2020-02-23)
5'-hydroxymethylcytosine (5hmC), an important 5'-cytosine modification, is altered highly in order in male meiotic prophase. However, the regulatory mechanism of this dynamic change and the function of 5hmC in meiosis remain largely unknown. Using a knockout mouse model, we showed
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