MABE143

Anti-Ago1 Antibody, clone 4G7-E12

Name: Anti-Ago1 Antibody, clone 4G7-E12
Brand: MM

clone 4G7-E12, from mouse

Synonym(s):

hAgo1, Argonaute1, eIF-2C 1, eIF2C 1, Q99, GERP95, DKFZp686M13167

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-Ago1 Antibody, clone 4G7-E12, clone 4G7-E12, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4G7-E12, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_036331

UniProt accession no.

Q9UL18

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... AGO1(26523)

Analysis Note

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Ago1 in HeLa cell lysate.

Application

Anti-Ago1 Antibody, clone 4G7-E12 is a Mouse Monoclonal Antibody for detection of Ago1 also known as hAgo1, Argonaute1, eIF-2C 1 & has been validated in WB.

Immunoprecipitation: A representative lot of this antibody was used for immunoprecipitation by an independent laboratory (see Azuma-Mukai A. et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).

General description

Ago1 (Eukaryotic translation initiation factor 2C 1) belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.

~97 kDa observed.

Immunogen

GST-tagged recombinant protein corresponding to human Ago1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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RNA-induced epigenetic silencing inhibits HIV-1 reactivation from latency.

Catalina Méndez et al.

Retrovirology, 15(1), 67-67 (2018-10-06)

Current antiretroviral therapy is effective in controlling HIV-1 infection. However, cessation of therapy is associated with rapid return of viremia from the viral reservoir. Eradicating the HIV-1 reservoir has proven difficult with the limited success of latency reactivation strategies and

The nuclear receptor ERβ engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading.

Roberta Tarallo et al.

Genome biology, 18(1), 189-189 (2017-10-12)

The RNA-binding protein Argonaute 2 (AGO2) is a key effector of RNA-silencing pathways It exerts a pivotal role in microRNA maturation and activity and can modulate chromatin remodeling, transcriptional gene regulation and RNA splicing. Estrogen receptor beta (ERβ) is endowed

Global Trade Item Number

SKU	GTIN
MABE143	04053252632853

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