MABE1664

Anti-INK4b (p15) Antibody, clone GF10 (10C7A6)

Name: Anti-INK4b (p15) Antibody, clone GF10 (10C7A6)
Brand: MM

culture supernatant, clone GF10 (10C7A6), from mouse

Synonym(s):

Cyclin-dependent kinase 4 inhibitor B, MTS-2, Multiple tumor suppressor 2, p14-INK4b, p15-INK4b, p15INK4B

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

GF10 (10C7A6), monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_004927

UniProt accession no.

P42772

target post-translational modification

unmodified

Gene Information

human ... CDKN2B(1030)

General description

Cyclin-dependent kinase 4 inhibitor B (UniProt P42772; also known as MTS-2, Multiple tumor suppressor 2, p14-INK4b, p15-INK4b, p15INK4B) is encoded by the CDKN2B (also known as MTS2) gene (Gene ID 1030) in human. The INK and CIP/KIP families of cyclin dependent kinase inhibitors (CDKis) protect cells from oncogenic signals-initiated cellular transformation. INK4a and INK4b (encoded by the CDKN2A and CDKN2B gene, respectively) constitute the INK4 subfamily of CDKis. INK4a and INK4b are induced by oncogenic Ras and are involved in cell cycle G1 arrest and cellular senescence. Inactivation of CDKN2A and CDKN2B are found in a wide variety of human malignancies and murine tumor models.

~15 kDa observed. 14.72 kDa (isoform 1; p15) and 8.078 kDa (isoform 2; p10) calculated.

Immunogen

Recombinant full-length human INK4b (p15).

Application

Anti-INK4b (p15) Antibody, clone GF10 (10C7A6) is an antibody against INK4b (p15) for use in Western Blotting.

Biochem/physiol Actions

Clone GF10 (10C7A6), Cat. No. MABE1664 and clone GF7 (7CIG4), Cat. No. 05-430 correspond to two independent clones originated from the same mouse host. Clone GF10 (10C7A6) is recommended as an alternative to clone GF7 (7CIG4), however these two clones are expected to recognize different epitopes, exhibit different target-binding affinity and species cross-reactivity. Cross-reactivity of clone GF10 (10C7A6) toward spliced isoform 2 (p10; UniProt P42772-2) has not been confirmed.

Physical form

Mouse monoclonal IgG1κ hybridoma culture supernatant with 0.05% sodium azide.

Analysis Note

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: A 1:250 dilution of this antibody detected INK4b (p15) in 50 µg of HEK293T cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Replaces: 05-430

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Inhibition of ERK1/2 in cancer-associated pancreatic stellate cells suppresses cancer-stromal interaction and metastasis.

Zilong Yan et al.

Journal of experimental & clinical cancer research : CR, 38(1), 221-221 (2019-05-28)

Extracellular signal-regulated kinases (ERKs) have been related to multiple cancers, including breast cancer, hepatocellular cancer, lung cancer and colorectal cancer. ERK1/2 inhibitor can suppress growth of KRAS-mutant pancreatic tumors by targeting cancer cell. However, no studies have shown the expression

Global Trade Item Number

SKU	GTIN
MABE1664	04055977350425

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