Anti-JMJD2A Antibody, clone 8B7.1, rabbit monoclonal

Jumonji domain-containing protein 2A (JMJD2A), or KDM4A, is a ubiquitously expressed demethylase enzyme, which belongs to the JMJD2 family. Like other members of this family, JMJD2A contains multiple domains: two Tudor domains, two PHD-type zinc fingers, one JmjC domain, and one JmjN domain. Little is known about the function of most of these domains; however, they are believed to be involved in transcriptional regulation. JMJD2A functions as a histone demethylase with specific activity against trimethylated Histone H3 Lysine residues Lys-9 and Lys-36. It has no effect on any other lysine residues, or on mono- and dimethylated Lys-9 and Lys-36. The demethylation of these residues induces the production of succinate and formaldehyde. Studies have shown that JMJD2A recruits NCOR1 and histone deacetylases, causing the respective transcriptional repression of E2Fand ASCL2 responsive promoters.

~120 kDa observed

KLH-conjugated linear peptide corresponding to human JMJD2A.

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected JMJD2A in human colorectal adenocarcinoma cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

This Anti-JMJD2A Antibody, clone 8B7.1 is validated for use in Western Blotting, IHC for the detection of JMJD2A.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
U2OS nuclear extract

Evaluated by Western Blot in U2OS nuclear extract.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected JMJD2A in 10 µg of U2OS nuclear extract.

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