Anti-dimethyl Histone H3 Antibody (Arg17), symmetric

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

~15 kDa observed

KLH-conjugated linear peptide corresponding to human symmetric Histone H3 dimethylated at Arg17.

Detect Histone H3 using this mouse monoclonal antibody, Anti-dimethyl Histone H3 Antibody (Arg17), symmetric validated for use in western blotting, Dot Blot & ICC.

Dot Blot Analysis: 1 µg/mL from a representative lot detected dimethyl Histone H3 (Arg17), symmetric in a panel of unmodified and modified Histones.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl Histone H3 (Arg17), symmetric in A431 and HepG2 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected dimethyl Histone H3 (Arg17), symmetric in 10 µg of HeLa nuclear extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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