Anti-PRMT1 Antibody, clone 7D2

The methylation of arginine is mediated by the Protein Arginine Methyl Transferase (PRMT) family of enzymes. These enzymes transfer the methyl group from S-adenosyl-Lmethionine to the guanidino nitrogen atoms of an arginine residue. Arginine methylation was found to be an important modification in signal transduction, transcription, RNA transport, and splicing. PRMT1, also known as HRMT1L2 and IR1B4, was isolated through its interaction with BTG1 and TIS21, proteins that are important in cell quiescence. PRMT1 is a 361 amino acid protein; its splicing variants differ at the N-terminus, giving rise to three isoforms of 343, 361, and 347 amino acids (variants 1, 2, and 3 respectively). Proteins involved in the metabolism of RNA are substrates for PRMT1, among them Sam68.

~ 42 kDa observed

BSA-conjugated linear peptide corresponding to human PRMT1.

Detect PRMT1 using this rat monoclonal antibody, Anti-PRMT1 Antibody & clone 7D2 validated for use in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Western Blotting Analysis: A representative lot from an independent laboratory detected PRMT1 in E. Coli containing untagged PRMT1, E.Coli extract containing His-tagged PRMT1, E.Coli extract containing GST- tagged PRMT1, and in DG75 whole cell extract (Gross, H., et al. (2012). PLoS ONE. 7(8):e42106.).

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HEK293 cell lysate.

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PRMT1 in 10 µg of HEK293 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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