Anti-phospho Histone H3 (Thr11) Antibody, clone N123/48

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation.

~17 kDa observed

Linear peptide corresponding to rat Histone H3 phosphorylated at Thr11.

Immmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected phospho Histone H3 (Thr11) in HeLa cells.

Dot Blot Analysis: A 1:5,000 dilution from a representative lot detected phospho Histone H3 (Thr11) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

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Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-phospho Histone H3 (Thr11) Antibody, clone N123/48 is validated for use in western blotting, ICC & dot blot for the detection of phospho Histone H3 (Thr11).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in Colcemid treated and untreated HeLa acid extract.

Western Blotting Anlaysis: 1:5,000 dilution of this antibody detected phospho Histone H3 (Thr11) in Colcemid treated HeLa acid extract and not in untreated HeLa acid extract.

Replaces: MABE468

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