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Key Documents

MABE441

Sigma-Aldrich

Anti-HJURP Antibody, clone 144K/B1

clone 144K/B1, from mouse

Synonym(s):

Holliday junction recognition protein, 14-3-3-associated AKT substrate, Fetal liver-expressing gene 1 protein, Up-regulated in lung cancer 9, HJURP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

144K/B1, monoclonal

species reactivity

human

technique(s)

ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_060880.3

UniProt accession no.

Q8NCD3

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HJURP(55355)

General description

Holliday junction recognition protein (HJURP) is also known as 14-3-3-associated AKT substrate (FAKTS), Fetal liver-expressing gene 1 protein (FLEG1) and Up-regulated in lung cancer 9 (URLC9). HJURP is a centromeric protein that acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. HJURP is highly expressed in the thymus, testis, and in many lung cancer samples with lower levels found in placenta, small intestine, liver, skeletal muscle, and colon.
~90 kDa observed

Immunogen

Recombinant protein corresponding to human HJURP.

Application

Detect Holliday junction recognition protein using this mouse monoclonal antibody, Anti-HJURP Antibody, clone 144K/B1 validated for use in western blotting & IP.
Immunoprecipitation Analysis: A representative lot immunoprecipitated HJURP in HeLa nuclear extract (Ana Losada, CNIO, Madrid, Spain).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot immmunoprecipitated HJURP in HEK293 cell lysate (Ana Losada, CNIO, Madrid, Spain).

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HJURP in 10 µg of HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Biao Huang et al.
iScience, 21, 375-390 (2019-11-11)
Epigenomic changes and stem cell deterioration are two hallmarks of aging. Accumulating evidence suggest that senescence of mesenchymal stromal cells (MSCs) perpetuates aging or age-related diseases. Here we report that two H3K9 demethylases, KDM3A and KDM4C, regulate heterochromatin reorganization via

Global Trade Item Number

SKUGTIN
MABE44104053252882913

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