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MABE455

Sigma-Aldrich

Anti-CHD-4 Antibody, clone 3F2/4

clone 3F2/4, from mouse

Synonym(s):

Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3F2/4, monoclonal

species reactivity

mouse, human

technique(s)

ChIP: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001264

UniProt accession no.

Q14839

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CHD4(1108)

General description

CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.
~220 kDa observed

Immunogen

Linear peptide corresponding to human CHD-4.

Application

Detect Chd4 (Chromodomain-helicase-DNA-binding protein 4) using this mouse monoclonal antibody, Anti-CHD-4 Antibody, clone 3F2/4 validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHD-4 in human breast carcinoma and skin tissues.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.

Immunofluorescence Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate
Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CHD-4 in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wei Shi et al.
Development (Cambridge, England), 151(8) (2024-04-15)
Regulation of chromatin states is essential for proper temporal and spatial gene expression. Chromatin states are modulated by remodeling complexes composed of components that have enzymatic activities. CHD4 is the catalytic core of the nucleosome remodeling and deacetylase (NuRD) complex

Global Trade Item Number

SKUGTIN
MABE45504053252920936

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