MABE635
Anti-acetyl-Histone H3 (Lys14) Antibody, clone RM130
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
antibody form
purified antibody
clone
RM130
monoclonal
purified by
using Protein A
species reactivity
rabbit, human
concentration
(Please refer to lot specific datasheet.)
technique(s)
multiplexing: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
General description
An linear peptide corresponding to human Histone H3 acetylated at Lys14.
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Application
Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
Biochem/physiol Actions
This antibody reacts to Histone H3 acetylated at Lysine 14 (K14ac), and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or Lysine 79 (K79ac) in Histone H3.
Physical form
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Evaluated by Western Blotting in untreated and sodium butyrate treated HeLa acid extract.
Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected acetyl-Histone H3 (Lys14) in sodium butyrate treated HeLa acid extract.
Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected acetyl-Histone H3 (Lys14) in sodium butyrate treated HeLa acid extract.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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