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Key Documents

MABE635

Sigma-Aldrich

Anti-acetyl-Histone H3 (Lys14) Antibody, clone RM130

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

antibody form

purified antibody

clone

RM130
monoclonal

purified by

using Protein A

species reactivity

rabbit, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

multiplexing: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

General description

An linear peptide corresponding to human Histone H3 acetylated at Lys14.
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Application

Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.

Biochem/physiol Actions

This antibody reacts to Histone H3 acetylated at Lysine 14 (K14ac), and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or Lysine 79 (K79ac) in Histone H3.

Physical form

Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Evaluated by Western Blotting in untreated and sodium butyrate treated HeLa acid extract.

Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected acetyl-Histone H3 (Lys14) in sodium butyrate treated HeLa acid extract.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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