MABE640

Anti-trimethyl Histone H3 (Lys36) Antibody, clone RM155

Name: Anti-trimethyl Histone H3 (Lys36) Antibody, clone RM155
Brand: MM

clone RM155, from rabbit

Synonym(s):

Histone H3.3, Histone H3

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-trimethyl Histone H3 (Lys36) Antibody, clone RM155, clone RM155, from rabbit

biological source

rabbit

antibody form

purified antibody

antibody product type

primary antibodies

clone

RM155, monoclonal

species reactivity

human

technique(s)

multiplexing: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

P84243

shipped in

wet ice

target post-translational modification

trimethylation (Lys36)

Quality Level

100

Gene Information

human ... H3F3B(3021)

Analysis Note

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis (WB): 2 μg/mL of this antibody detected trimethyl Histone H3 (Lys36) in Recombinant HeLa protein and HeLa acid extract.

Application

This Anti-trimethyl Histone H3 (Lys36) Antibody, clone RM155 is validated for use in Western Blotting, Multiplexing for the detection of trimethyl Histone H3.

Biochem/physiol Actions

This antibody reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with monomethylated Lysine 36 (K36me1), dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.

General description

Information Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.

~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Immunogen

A linear peptide corresponding to human Histone H3 trimethylated at Lys36.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

Global Trade Item Number

SKU	GTIN
MABE640	04055977181425

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