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MABE869

Sigma-Aldrich

Anti-Lin28A Antibody, clone 2J3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

conjugate

unconjugated

Quality Level

antibody form

purified antibody

clone

2J3
monoclonal

purified by

using protein G

species reactivity

mouse, human, mouse

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

target post-translational modification

unmodified

General description

Recombinant protein corresponding to human Lin28A.
~26 kDa observed

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28A in A3-1 mouse embryonic stem cells (Narry Kim, Seoul National University).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28A in A3-1 mouse embryonic stem cells (Cho, J., et al. (2012) Cell. 151:765-777).
CLIP-seq Analysis: A representative lot immunoprecipitated Lin28A in A3-1 mouse embryonic stem cells (Cho, J., et al. (2012) Cell. 151:765-777).
Western Blotting Analysis: A representative lot detected Lin28A in A3-1 mouse embryonic stem cells (Narry Kim, Seoul National University).

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in NTera2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Lin28A in 10 µg of NTera2 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MABE86904055977135312

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